Immunogenetic responses in calves to intranasal delivery of bovine respiratory syncytial virus (BRSV) epitopes encapsulated in poly (DL-lactide-co-glycolide) microparticles

Owen V Kavanagh, Brian M Adair, Michael Welsh, Bernadette Earley

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bovine respiratory syncytial virus (BRSV) is the principal aetiological agent of the bovine respiratory disease complex. A BRSV subunit vaccine candidate consisting of two synthetic peptides representing putative protective epitopes on BRSV surface glycoproteins in soluble form or encapsulated in poly(lactide-co-glycolide) (PLG) microparticles were prepared. Calves (10 weeks old) with diminishing levels of BRSV-specific maternal antibody were intranasally administered a single dose of the different peptide formulations. Peptide-specific local immune responses (nasal secretion IgA), but not systemic humoral (serum IgG) or cellular responses (serum IFN-γ), were generated by all forms of peptide. There was a significant reduction in occurrence of respiratory disease in the animals inoculated with all peptide formulations compared to animals given PBS alone. Furthermore no adverse effects were observed in any of the animals post vaccination. These results suggest that intranasal immunisation with the peptide subunit vaccine does induce an as yet unidentified protective immune response.

Original languageEnglish
Pages (from-to)786-93
Number of pages8
JournalResearch in Veterinary Science
Volume95
Issue number2
DOIs
Publication statusPublished - Oct 2013

Bibliographical note

Copyright © 2013 Elsevier Ltd. All rights reserved.

Keywords

  • Administration, Intranasal
  • Aging
  • Animals
  • Antibodies, Viral
  • Cattle
  • Epitopes
  • Female
  • Immunity, Maternally-Acquired
  • Lactic Acid
  • Particle Size
  • Polyglycolic Acid
  • Respiratory Syncytial Virus Infections
  • Respiratory Syncytial Virus, Bovine
  • Viral Vaccines

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