Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics

James Peter Stewart; Jana Gazdova; Shambhavi Srivastava; Julia Revolta; Louise Harewood; Manisha Maurya; Nikos Darzentas; David Gonzalez

doi:10.1007/978-1-0716-2115-8_9

Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics

James Peter Stewart, Jana Gazdova, Shambhavi Srivastava, Julia Revolta, Louise Harewood, Manisha Maurya, Nikos Darzentas, David Gonzalez

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

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Abstract

In the era of genomic medicine, targeted next generation sequencing strategies (NGS) are becoming increasingly adopted by clinical molecular diagnostic laboratories to identify genetic diagnostic and prognostic biomarkers in hemato-oncology. We describe the EuroClonality-NGS DNA Capture (EuroClonality-NDC) assay, which is designed to simultaneously detect B and T cell clonal rearrangements, translocations, copy number alterations, and sequence variants. The accompanying validated bioinformatics pipeline enables production of an integrated report. The combination of the laboratory protocol and bioinformatics pipeline in the EuroClonality-NDC minimizes the potential for human error, reduces economic costs compared to current molecular testing strategies, and should improve diagnostic outcomes.

Original language	English
Title of host publication	Immunogenetics. Methods and Protocols
Editors	Anton W. Langerak
Publisher	Springer
Pages	133-152
Number of pages	20
ISBN (Electronic)	978-1-0716-2115-8
DOIs	https://doi.org/10.1007/978-1-0716-2115-8_9
Publication status	Published - 28 May 2022

Publication series

Name	Methods in molecular biology (Clifton, N.J.)
Publisher	Humana Press
Volume	2453
ISSN (Print)	1064-3745

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10.1007/978-1-0716-2115-8_9Licence: CC BY

Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics
Copyright 2022 the authors. This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)
Final published version, 312 KBLicence: CC BY

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Stewart, J. P., Gazdova, J., Srivastava, S., Revolta, J., Harewood, L., Maurya, M., Darzentas, N., & Gonzalez, D. (2022). Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics. In A. W. Langerak (Ed.), Immunogenetics. Methods and Protocols (pp. 133-152). (Methods in molecular biology (Clifton, N.J.); Vol. 2453). Springer. https://doi.org/10.1007/978-1-0716-2115-8_9

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abstract = "In the era of genomic medicine, targeted next generation sequencing strategies (NGS) are becoming increasingly adopted by clinical molecular diagnostic laboratories to identify genetic diagnostic and prognostic biomarkers in hemato-oncology. We describe the EuroClonality-NGS DNA Capture (EuroClonality-NDC) assay, which is designed to simultaneously detect B and T cell clonal rearrangements, translocations, copy number alterations, and sequence variants. The accompanying validated bioinformatics pipeline enables production of an integrated report. The combination of the laboratory protocol and bioinformatics pipeline in the EuroClonality-NDC minimizes the potential for human error, reduces economic costs compared to current molecular testing strategies, and should improve diagnostic outcomes.",

author = "Stewart, {James Peter} and Jana Gazdova and Shambhavi Srivastava and Julia Revolta and Louise Harewood and Manisha Maurya and Nikos Darzentas and David Gonzalez",

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Stewart, JP, Gazdova, J, Srivastava, S, Revolta, J, Harewood, L, Maurya, M, Darzentas, N & Gonzalez, D 2022, Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics. in AW Langerak (ed.), Immunogenetics. Methods and Protocols . Methods in molecular biology (Clifton, N.J.), vol. 2453, Springer, pp. 133-152. https://doi.org/10.1007/978-1-0716-2115-8_9

Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics. / Stewart, James Peter; Gazdova, Jana; Srivastava, Shambhavi et al.
Immunogenetics. Methods and Protocols . ed. / Anton W. Langerak. Springer, 2022. p. 133-152 (Methods in molecular biology (Clifton, N.J.); Vol. 2453).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics

AU - Stewart, James Peter

AU - Gazdova, Jana

AU - Srivastava, Shambhavi

AU - Revolta, Julia

AU - Harewood, Louise

AU - Maurya, Manisha

AU - Darzentas, Nikos

AU - Gonzalez, David

PY - 2022/5/28

Y1 - 2022/5/28

N2 - In the era of genomic medicine, targeted next generation sequencing strategies (NGS) are becoming increasingly adopted by clinical molecular diagnostic laboratories to identify genetic diagnostic and prognostic biomarkers in hemato-oncology. We describe the EuroClonality-NGS DNA Capture (EuroClonality-NDC) assay, which is designed to simultaneously detect B and T cell clonal rearrangements, translocations, copy number alterations, and sequence variants. The accompanying validated bioinformatics pipeline enables production of an integrated report. The combination of the laboratory protocol and bioinformatics pipeline in the EuroClonality-NDC minimizes the potential for human error, reduces economic costs compared to current molecular testing strategies, and should improve diagnostic outcomes.

AB - In the era of genomic medicine, targeted next generation sequencing strategies (NGS) are becoming increasingly adopted by clinical molecular diagnostic laboratories to identify genetic diagnostic and prognostic biomarkers in hemato-oncology. We describe the EuroClonality-NGS DNA Capture (EuroClonality-NDC) assay, which is designed to simultaneously detect B and T cell clonal rearrangements, translocations, copy number alterations, and sequence variants. The accompanying validated bioinformatics pipeline enables production of an integrated report. The combination of the laboratory protocol and bioinformatics pipeline in the EuroClonality-NDC minimizes the potential for human error, reduces economic costs compared to current molecular testing strategies, and should improve diagnostic outcomes.

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M3 - Chapter (peer-reviewed)

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T3 - Methods in molecular biology (Clifton, N.J.)

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BT - Immunogenetics. Methods and Protocols

A2 - Langerak, Anton W.

PB - Springer

ER -

Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics

Abstract

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