Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics

James Peter Stewart, Jana Gazdova, Shambhavi Srivastava, Julia Revolta, Louise Harewood, Manisha Maurya, Nikos Darzentas, David Gonzalez

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

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Abstract

In the era of genomic medicine, targeted next generation sequencing strategies (NGS) are becoming increasingly adopted by clinical molecular diagnostic laboratories to identify genetic diagnostic and prognostic biomarkers in hemato-oncology. We describe the EuroClonality-NGS DNA Capture (EuroClonality-NDC) assay, which is designed to simultaneously detect B and T cell clonal rearrangements, translocations, copy number alterations, and sequence variants. The accompanying validated bioinformatics pipeline enables production of an integrated report. The combination of the laboratory protocol and bioinformatics pipeline in the EuroClonality-NDC minimizes the potential for human error, reduces economic costs compared to current molecular testing strategies, and should improve diagnostic outcomes.

Original languageEnglish
Title of host publicationImmunogenetics. Methods and Protocols
EditorsAnton W. Langerak
PublisherSpringer
Pages133-152
Number of pages20
ISBN (Electronic)978-1-0716-2115-8
DOIs
Publication statusPublished - 28 May 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
Volume2453
ISSN (Print)1064-3745

Bibliographical note

© 2022. The Author(s).

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