Immunolocalisation of the glutathione s-transferases, GST-26 and GST-28, within adult schistosoma japonicum

G. N. Gobert; D. J. Stenzel; D. P. McManus

doi:10.1016/S0020-7519(98)00115-5

Immunolocalisation of the glutathione s-transferases, GST-26 and GST-28, within adult schistosoma japonicum

G. N. Gobert
, D. J. Stenzel^*
, D. P. McManus

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

This paper describes the first ultrastructural immunolocalisation study of the 26-kDa and 28-kDa glutathione S-transferases within adult Schistosoma japonicum (GST-26 and GST-28). Polyclonal antibodies raised against GST-28 (in mice) and against GST-26 (in rabbits) were used to examine the distribution of the proteins within adult parasites. Both proteins were localised within the parenchymal region of the male parasite. Additionally, both proteins were present within parenchymal cells located between the vitelline glands of female parasites. There were no detectable levels of GST-26 or GST-28 on the surface or within the tegument matrix of either the male or female worms. Possible functions for GST-26 and GST-28 within S. japonicum and their significance as vaccine target molecules are addressed.

Original language	English
Pages (from-to)	1437-1443
Number of pages	7
Journal	International Journal for Parasitology
Volume	28
Issue number	9
DOIs	https://doi.org/10.1016/S0020-7519(98)00115-5
Publication status	Published - 01 Sept 1998
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Glutathione S-transferase
Immunolocalisation
Schistosoma japonicum

ASJC Scopus subject areas

Parasitology
Infectious Diseases

Access to Document

10.1016/S0020-7519(98)00115-5

Cite this

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title = "Immunolocalisation of the glutathione s-transferases, GST-26 and GST-28, within adult schistosoma japonicum",

abstract = "This paper describes the first ultrastructural immunolocalisation study of the 26-kDa and 28-kDa glutathione S-transferases within adult Schistosoma japonicum (GST-26 and GST-28). Polyclonal antibodies raised against GST-28 (in mice) and against GST-26 (in rabbits) were used to examine the distribution of the proteins within adult parasites. Both proteins were localised within the parenchymal region of the male parasite. Additionally, both proteins were present within parenchymal cells located between the vitelline glands of female parasites. There were no detectable levels of GST-26 or GST-28 on the surface or within the tegument matrix of either the male or female worms. Possible functions for GST-26 and GST-28 within S. japonicum and their significance as vaccine target molecules are addressed.",

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author = "Gobert, \{G. N.\} and Stenzel, \{D. J.\} and McManus, \{D. P.\}",

year = "1998",

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doi = "10.1016/S0020-7519(98)00115-5",

language = "English",

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TY - JOUR

T1 - Immunolocalisation of the glutathione s-transferases, GST-26 and GST-28, within adult schistosoma japonicum

AU - Gobert, G. N.

AU - Stenzel, D. J.

AU - McManus, D. P.

PY - 1998/9/1

Y1 - 1998/9/1

N2 - This paper describes the first ultrastructural immunolocalisation study of the 26-kDa and 28-kDa glutathione S-transferases within adult Schistosoma japonicum (GST-26 and GST-28). Polyclonal antibodies raised against GST-28 (in mice) and against GST-26 (in rabbits) were used to examine the distribution of the proteins within adult parasites. Both proteins were localised within the parenchymal region of the male parasite. Additionally, both proteins were present within parenchymal cells located between the vitelline glands of female parasites. There were no detectable levels of GST-26 or GST-28 on the surface or within the tegument matrix of either the male or female worms. Possible functions for GST-26 and GST-28 within S. japonicum and their significance as vaccine target molecules are addressed.

AB - This paper describes the first ultrastructural immunolocalisation study of the 26-kDa and 28-kDa glutathione S-transferases within adult Schistosoma japonicum (GST-26 and GST-28). Polyclonal antibodies raised against GST-28 (in mice) and against GST-26 (in rabbits) were used to examine the distribution of the proteins within adult parasites. Both proteins were localised within the parenchymal region of the male parasite. Additionally, both proteins were present within parenchymal cells located between the vitelline glands of female parasites. There were no detectable levels of GST-26 or GST-28 on the surface or within the tegument matrix of either the male or female worms. Possible functions for GST-26 and GST-28 within S. japonicum and their significance as vaccine target molecules are addressed.

KW - Glutathione S-transferase

KW - Immunolocalisation

KW - Schistosoma japonicum

UR - https://www.scopus.com/pages/publications/0031867428

U2 - 10.1016/S0020-7519(98)00115-5

DO - 10.1016/S0020-7519(98)00115-5

M3 - Article

C2 - 9770630

AN - SCOPUS:0031867428

SN - 0020-7519

VL - 28

SP - 1437

EP - 1443

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 9

ER -

Immunolocalisation of the glutathione s-transferases, GST-26 and GST-28, within adult schistosoma japonicum

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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