Immunolocalization of the fatty acid-binding protein Sj-FABPc within adult Schistosoma japonicum

G. N. Gobert; D. J. Stenzel; M. K. Jones; D. P. McManus

doi:10.1017/S0031182097008925

Immunolocalization of the fatty acid-binding protein Sj-FABPc within adult Schistosoma japonicum

G. N. Gobert^*, D. J. Stenzel, M. K. Jones, D. P. McManus

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

This paper describes the first localization study of the 14.7 kDa fatty acid-binding protein in Schistosoma japonicum (Sj-FABPc) using transmission electron microscopy. A polyclonal antibody raised against recombinant Sj- FABPc was used in combination with a colloidal gold marker to determine the distribution of the protein within adult parasites. Sj-FABPc was localized within lipid droplets below the subtegumental region of the male parasite. Additionally, Sj-FABPc was present in the vitelline droplets of the vitelline glands of female parasites. There were no detectable levels of Sj-FABPc on the surface or within the tegument of male or female parasites. Possible functions of Sj-FABPc within S. japonicum and the relevance of these immunolocalization findings in light of the recent reports that the homologue Sm-FABPc is an important anti-S. mansoni vaccine target molecule are also discussed.

Original language	English
Pages (from-to)	33-39
Number of pages	7
Journal	Parasitology
Volume	115
Issue number	1
DOIs	https://doi.org/10.1017/S0031182097008925
Publication status	Published - 22 Aug 1997
Externally published	Yes

Keywords

Cryopreparation
Electron microscopy
FABPc
Immunolocalization
Schistosoma japonicum
Vitelline glands

ASJC Scopus subject areas

Parasitology
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1017/S0031182097008925

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title = "Immunolocalization of the fatty acid-binding protein Sj-FABPc within adult Schistosoma japonicum",

abstract = "This paper describes the first localization study of the 14.7 kDa fatty acid-binding protein in Schistosoma japonicum (Sj-FABPc) using transmission electron microscopy. A polyclonal antibody raised against recombinant Sj- FABPc was used in combination with a colloidal gold marker to determine the distribution of the protein within adult parasites. Sj-FABPc was localized within lipid droplets below the subtegumental region of the male parasite. Additionally, Sj-FABPc was present in the vitelline droplets of the vitelline glands of female parasites. There were no detectable levels of Sj-FABPc on the surface or within the tegument of male or female parasites. Possible functions of Sj-FABPc within S. japonicum and the relevance of these immunolocalization findings in light of the recent reports that the homologue Sm-FABPc is an important anti-S. mansoni vaccine target molecule are also discussed.",

keywords = "Cryopreparation, Electron microscopy, FABPc, Immunolocalization, Schistosoma japonicum, Vitelline glands",

author = "Gobert, {G. N.} and Stenzel, {D. J.} and Jones, {M. K.} and McManus, {D. P.}",

year = "1997",

month = aug,

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doi = "10.1017/S0031182097008925",

language = "English",

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pages = "33--39",

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AU - Gobert, G. N.

AU - Stenzel, D. J.

AU - Jones, M. K.

AU - McManus, D. P.

PY - 1997/8/22

Y1 - 1997/8/22

N2 - This paper describes the first localization study of the 14.7 kDa fatty acid-binding protein in Schistosoma japonicum (Sj-FABPc) using transmission electron microscopy. A polyclonal antibody raised against recombinant Sj- FABPc was used in combination with a colloidal gold marker to determine the distribution of the protein within adult parasites. Sj-FABPc was localized within lipid droplets below the subtegumental region of the male parasite. Additionally, Sj-FABPc was present in the vitelline droplets of the vitelline glands of female parasites. There were no detectable levels of Sj-FABPc on the surface or within the tegument of male or female parasites. Possible functions of Sj-FABPc within S. japonicum and the relevance of these immunolocalization findings in light of the recent reports that the homologue Sm-FABPc is an important anti-S. mansoni vaccine target molecule are also discussed.

AB - This paper describes the first localization study of the 14.7 kDa fatty acid-binding protein in Schistosoma japonicum (Sj-FABPc) using transmission electron microscopy. A polyclonal antibody raised against recombinant Sj- FABPc was used in combination with a colloidal gold marker to determine the distribution of the protein within adult parasites. Sj-FABPc was localized within lipid droplets below the subtegumental region of the male parasite. Additionally, Sj-FABPc was present in the vitelline droplets of the vitelline glands of female parasites. There were no detectable levels of Sj-FABPc on the surface or within the tegument of male or female parasites. Possible functions of Sj-FABPc within S. japonicum and the relevance of these immunolocalization findings in light of the recent reports that the homologue Sm-FABPc is an important anti-S. mansoni vaccine target molecule are also discussed.

KW - Cryopreparation

KW - Electron microscopy

KW - FABPc

KW - Immunolocalization

KW - Schistosoma japonicum

KW - Vitelline glands

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DO - 10.1017/S0031182097008925

M3 - Article

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AN - SCOPUS:0030804974

SN - 0031-1820

VL - 115

SP - 33

EP - 39

JO - Parasitology

JF - Parasitology

IS - 1

ER -

Immunolocalization of the fatty acid-binding protein Sj-FABPc within adult Schistosoma japonicum

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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