Immunolocalization of the fatty acid-binding protein Sj-FABPc within adult Schistosoma japonicum

G. N. Gobert*, D. J. Stenzel, M. K. Jones, D. P. McManus

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This paper describes the first localization study of the 14.7 kDa fatty acid-binding protein in Schistosoma japonicum (Sj-FABPc) using transmission electron microscopy. A polyclonal antibody raised against recombinant Sj- FABPc was used in combination with a colloidal gold marker to determine the distribution of the protein within adult parasites. Sj-FABPc was localized within lipid droplets below the subtegumental region of the male parasite. Additionally, Sj-FABPc was present in the vitelline droplets of the vitelline glands of female parasites. There were no detectable levels of Sj-FABPc on the surface or within the tegument of male or female parasites. Possible functions of Sj-FABPc within S. japonicum and the relevance of these immunolocalization findings in light of the recent reports that the homologue Sm-FABPc is an important anti-S. mansoni vaccine target molecule are also discussed.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalParasitology
Volume115
Issue number1
DOIs
Publication statusPublished - 22 Aug 1997
Externally publishedYes

Keywords

  • Cryopreparation
  • Electron microscopy
  • FABPc
  • Immunolocalization
  • Schistosoma japonicum
  • Vitelline glands

ASJC Scopus subject areas

  • Parasitology
  • Immunology

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