Immunological homologues of the Arabidopsis thaliana beta 1 tubulin are polyglutamylated in Nicotiana tabacum

AP Smertenko, SL Lawrence, PJ Hussey*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Peptide-specific antibody AABI, raised to the C-terminal 13 amino acids of Arabidopsis thaliana beta 1 tubulin, identifies a single electrophoretically separable beta-tubulin on 2-D-gel Western blots of total protein extracts from A. thaliana seedlings. We show that AABI crossreacts with two of the eight polyglutamylated beta-tubulin isoforms present in purified Nicotiana tabacum tubulin fractionated by high-resolution isoelectric focussing. Immunolocalisation studies using AAB1 revealed that the two N. tabacum polyglutamylated beta 1-tubulin isoforms are utilised in all four plant microtubule arrays (the interphase cortical array, the preprophase band, the spindle and the phragmoplast) indicating that there is no apparent subcellular sorting of these isotypes.

Original languageEnglish
Pages (from-to)138-143
Number of pages6
JournalProtoplasma
Volume203
Issue number3-4
Publication statusPublished - 1998

Keywords

  • MICROTUBULES
  • ISOFORMS
  • SMALL GENOME
  • polyglutamylation
  • CELLS
  • Nicotiana tabacum
  • ALPHA-TUBULIN
  • POSTTRANSLATIONAL MODIFICATIONS
  • ISOTYPES
  • PLANT
  • Arabidopsis thaliana
  • beta-tubulin isoforms
  • IDENTIFICATION
  • ANTIBODIES
  • plant tubulin

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