Impact of fractionation on out-of-field survival and DNA damage responses following exposure to intensity modulated radiation fields

Mihaela Ghita, Caroline B. Coffey, Karl T Butterworth, Stephen J McMahon, Giuseppe Schettino, Kevin M Prise

Research output: Contribution to journalArticle

5 Citations (Scopus)
185 Downloads (Pure)

Abstract

To limit toxicity to normal tissues adjacent to the target tumour volume, radiotherapy is delivered using fractionated regimes whereby the total prescribed dose is given as a series of sequential smaller doses separated by specific time intervals. The impact of fractionation on out-of-field survival and DNA damage responses was determined in AGO-1522 primary human fibroblasts and MCF-7 breast tumour cells using uniform and modulated exposures delivered using a 225 kVp x-ray source. Responses to fractionated schedules (two equal fractions delivered with time intervals from 4 h to 48 h) were compared to those following acute exposures. Cell survival and DNA damage repair measurements indicate that cellular responses to fractionated non-uniform exposures differ from those seen in uniform exposures for the investigated cell lines. Specifically, there is a consistent lack of repair observed in the out-of-field populations during intervals between fractions, confirming the importance of cell signalling to out-of-field responses in a fractionated radiation schedule, and this needs to be confirmed for a wider range of cell lines and conditions.

Original languageEnglish
Pages (from-to)515-526
Number of pages12
JournalPhysics in Medicine and Biology
Volume61
Issue number2
Early online date18 Dec 2015
DOIs
Publication statusPublished - 21 Jan 2016

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