Impact of Sample Heterogeneity on Methylation Analysis

M. Loh, N. Liem, P.L. Lim, A. Vaithilingam, C.L. Cheng, Manuel Salto-Tellez, W.P. Yong, R. Soong

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


The recent emergence of high-throughput arrays for methylation analysis has made the influence of tumor content on the interpretation of methylation levels increasingly pertinent. However, to what degree does tumor content have an influence, and what degree of tumor content makes a specimen acceptable for accurate analysis remains unclear. Taking a systematic approach, we analyzed 98 unselected formalin-fixed and paraffin-embedded gastric tumors and matched normal tissue samples using the Illumina GoldenGate methylation assay. Unsupervised hierarchical clustering showed 2 separate clusters with a significant difference in average tumor content levels. The probes identified to be significantly differentially methylated between the tumors and normals also differed according to the tumor content of the samples included, with the sensitivity of identifying the
Original languageEnglish
Pages (from-to)243-247
Number of pages5
JournalDiagnostic Molecular Pathology
Issue number4
Publication statusPublished - Dec 2010

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology


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