Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches

Irene R Grant; Linda D Stewart

doi:10.1007/978-1-4939-2004-4_11

Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches

Irene R Grant^*, Linda D Stewart

^*Corresponding author for this work

School of Biological Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

2 Citations (Scopus)

Abstract

Immunomagnetic separation (IMS) represents a simple but effective method of selectively capturing and concentrating Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), from tissue samples. It is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. IMS is performed with paramagnetic beads coated with M. bovis-specific antibody and peptide binders. Once captured by IMS, M. bovis cells can be detected by either PCR or cultural detection methods. Increased detection rates of M. bovis, particularly from non-visibly lesioned lymph node tissues from bTB reactor animals, have recently been reported when IMS-based methods were employed.

Original language	English
Title of host publication	Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies
Subtitle of host publication	Molecular Diagnostics and High-Throughput Strategies
Place of Publication	New Jersey, USA
Publisher	Springer New York
Pages	153-161
Number of pages	9
Volume	1247
ISBN (Electronic)	978-1-4939-2004-4
ISBN (Print)	978-1-4939-2003-7
DOIs	https://doi.org/10.1007/978-1-4939-2004-4_11
Publication status	Published - Jan 2015

Publication series

Name	Methods in Molecular Biology
Volume	1247

Keywords

Detection
Immunomagnetic separation (IMS)
IMS-culture
IMS-PCR
Isolation
Lymph node tissue
Mycobacterium bovis

ASJC Scopus subject areas

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-2004-4_11

http://www.springer.com/medicine/internal/book/978-1-4939-2003-7

Cite this

Grant, I. R., & Stewart, L. D. (2015). Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches. In Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies: Molecular Diagnostics and High-Throughput Strategies (Vol. 1247, pp. 153-161). (Methods in Molecular Biology; Vol. 1247). Springer New York. https://doi.org/10.1007/978-1-4939-2004-4_11

Grant, Irene R ; Stewart, Linda D. / Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches. Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies: Molecular Diagnostics and High-Throughput Strategies. Vol. 1247 New Jersey, USA : Springer New York, 2015. pp. 153-161 (Methods in Molecular Biology).

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abstract = "Immunomagnetic separation (IMS) represents a simple but effective method of selectively capturing and concentrating Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), from tissue samples. It is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. IMS is performed with paramagnetic beads coated with M. bovis-specific antibody and peptide binders. Once captured by IMS, M. bovis cells can be detected by either PCR or cultural detection methods. Increased detection rates of M. bovis, particularly from non-visibly lesioned lymph node tissues from bTB reactor animals, have recently been reported when IMS-based methods were employed.",

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Grant, IR & Stewart, LD 2015, Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches. in Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies: Molecular Diagnostics and High-Throughput Strategies. vol. 1247, Methods in Molecular Biology, vol. 1247, Springer New York, New Jersey, USA, pp. 153-161. https://doi.org/10.1007/978-1-4939-2004-4_11

Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches. / Grant, Irene R ; Stewart, Linda D.
Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies: Molecular Diagnostics and High-Throughput Strategies. Vol. 1247 New Jersey, USA: Springer New York, 2015. p. 153-161 (Methods in Molecular Biology; Vol. 1247).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches

AU - Grant, Irene R

AU - Stewart, Linda D

PY - 2015/1

Y1 - 2015/1

N2 - Immunomagnetic separation (IMS) represents a simple but effective method of selectively capturing and concentrating Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), from tissue samples. It is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. IMS is performed with paramagnetic beads coated with M. bovis-specific antibody and peptide binders. Once captured by IMS, M. bovis cells can be detected by either PCR or cultural detection methods. Increased detection rates of M. bovis, particularly from non-visibly lesioned lymph node tissues from bTB reactor animals, have recently been reported when IMS-based methods were employed.

AB - Immunomagnetic separation (IMS) represents a simple but effective method of selectively capturing and concentrating Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), from tissue samples. It is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. IMS is performed with paramagnetic beads coated with M. bovis-specific antibody and peptide binders. Once captured by IMS, M. bovis cells can be detected by either PCR or cultural detection methods. Increased detection rates of M. bovis, particularly from non-visibly lesioned lymph node tissues from bTB reactor animals, have recently been reported when IMS-based methods were employed.

KW - Detection

KW - Immunomagnetic separation (IMS)

KW - IMS-culture

KW - IMS-PCR

KW - Isolation

KW - Lymph node tissue

KW - Mycobacterium bovis

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U2 - 10.1007/978-1-4939-2004-4_11

DO - 10.1007/978-1-4939-2004-4_11

M3 - Chapter (peer-reviewed)

C2 - 25399094

SN - 978-1-4939-2003-7

VL - 1247

T3 - Methods in Molecular Biology

SP - 153

EP - 161

BT - Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies

PB - Springer New York

CY - New Jersey, USA

ER -

Grant IR , Stewart LD. Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches. In Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies: Molecular Diagnostics and High-Throughput Strategies. Vol. 1247. New Jersey, USA: Springer New York. 2015. p. 153-161. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-2004-4_11

Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

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