Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches

Irene R Grant*, Linda D Stewart

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

2 Citations (Scopus)

Abstract

Immunomagnetic separation (IMS) represents a simple but effective method of selectively capturing and concentrating Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), from tissue samples. It is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. IMS is performed with paramagnetic beads coated with M. bovis-specific antibody and peptide binders. Once captured by IMS, M. bovis cells can be detected by either PCR or cultural detection methods. Increased detection rates of M. bovis, particularly from non-visibly lesioned lymph node tissues from bTB reactor animals, have recently been reported when IMS-based methods were employed.

Original languageEnglish
Title of host publicationVeterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies
Subtitle of host publicationMolecular Diagnostics and High-Throughput Strategies
Place of PublicationNew Jersey, USA
PublisherSpringer New York
Pages153-161
Number of pages9
Volume1247
ISBN (Electronic)978-1-4939-2004-4
ISBN (Print)978-1-4939-2003-7
DOIs
Publication statusPublished - Jan 2015

Publication series

NameMethods in Molecular Biology
Volume1247

Keywords

  • Detection
  • Immunomagnetic separation (IMS)
  • IMS-culture
  • IMS-PCR
  • Isolation
  • Lymph node tissue
  • Mycobacterium bovis

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Grant, I. R., & Stewart, L. D. (2015). Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches. In Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies: Molecular Diagnostics and High-Throughput Strategies (Vol. 1247, pp. 153-161). (Methods in Molecular Biology; Vol. 1247). Springer New York. https://doi.org/10.1007/978-1-4939-2004-4_11