In situ hybridization for the detection of chicken anemia virus in formalin-fixed, paraffin-embedded sections.

G. M. Allan; J. A. Smyth; D. Todd; M. S. McNulty

doi:10.2307/1591471

In situ hybridization for the detection of chicken anemia virus in formalin-fixed, paraffin-embedded sections.

G. M. Allan^*, J. A. Smyth, D. Todd, M. S. McNulty

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The development of a biotinylated in situ hybridization procedure for the detection of chicken anemia virus (CAV) is described. A double-stranded DNA probe was prepared using polymerase chain reaction and was biotinylated by nick translation. Hybridization conditions included the use of a microwave oven to denature target and probe nucleic acids and a five-step protocol for detection of biotinylated hybrids. In situ hybridization detected CAV nucleic acid in thymus tissue from experimentally infected birds after 6 hours, 3 days, and 7 days fixation time in formalin. However, immunocytochemical detection of CAV antigen was severely impaired if tissue was fixed in formalin for more than 6 hours.

Original language	English
Pages (from-to)	177-182
Number of pages	6
Journal	Avian Diseases
Volume	37
Issue number	1
DOIs	https://doi.org/10.2307/1591471
Publication status	Published - 1993
Externally published	Yes

Bibliographical note

Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

ASJC Scopus subject areas

Food Animals
Animal Science and Zoology
Immunology and Microbiology(all)

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title = "In situ hybridization for the detection of chicken anemia virus in formalin-fixed, paraffin-embedded sections.",

abstract = "The development of a biotinylated in situ hybridization procedure for the detection of chicken anemia virus (CAV) is described. A double-stranded DNA probe was prepared using polymerase chain reaction and was biotinylated by nick translation. Hybridization conditions included the use of a microwave oven to denature target and probe nucleic acids and a five-step protocol for detection of biotinylated hybrids. In situ hybridization detected CAV nucleic acid in thymus tissue from experimentally infected birds after 6 hours, 3 days, and 7 days fixation time in formalin. However, immunocytochemical detection of CAV antigen was severely impaired if tissue was fixed in formalin for more than 6 hours.",

author = "Allan, {G. M.} and Smyth, {J. A.} and D. Todd and McNulty, {M. S.}",

note = "Copyright: This record is sourced from MEDLINE{\textregistered}/PubMed{\textregistered}, a database of the U.S. National Library of Medicine",

year = "1993",

doi = "10.2307/1591471",

language = "English",

volume = "37",

pages = "177--182",

journal = "Avian Diseases",

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publisher = "American Association of Avian Pathologists",

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T1 - In situ hybridization for the detection of chicken anemia virus in formalin-fixed, paraffin-embedded sections.

AU - Allan, G. M.

AU - Smyth, J. A.

AU - Todd, D.

AU - McNulty, M. S.

N1 - Copyright: This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

PY - 1993

Y1 - 1993

N2 - The development of a biotinylated in situ hybridization procedure for the detection of chicken anemia virus (CAV) is described. A double-stranded DNA probe was prepared using polymerase chain reaction and was biotinylated by nick translation. Hybridization conditions included the use of a microwave oven to denature target and probe nucleic acids and a five-step protocol for detection of biotinylated hybrids. In situ hybridization detected CAV nucleic acid in thymus tissue from experimentally infected birds after 6 hours, 3 days, and 7 days fixation time in formalin. However, immunocytochemical detection of CAV antigen was severely impaired if tissue was fixed in formalin for more than 6 hours.

AB - The development of a biotinylated in situ hybridization procedure for the detection of chicken anemia virus (CAV) is described. A double-stranded DNA probe was prepared using polymerase chain reaction and was biotinylated by nick translation. Hybridization conditions included the use of a microwave oven to denature target and probe nucleic acids and a five-step protocol for detection of biotinylated hybrids. In situ hybridization detected CAV nucleic acid in thymus tissue from experimentally infected birds after 6 hours, 3 days, and 7 days fixation time in formalin. However, immunocytochemical detection of CAV antigen was severely impaired if tissue was fixed in formalin for more than 6 hours.

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DO - 10.2307/1591471

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AN - SCOPUS:0027350330

VL - 37

SP - 177

EP - 182

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 1

ER -

In situ hybridization for the detection of chicken anemia virus in formalin-fixed, paraffin-embedded sections.

Abstract

Bibliographical note

ASJC Scopus subject areas

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