In situ hybridization for the detection of chicken anemia virus in formalin-fixed, paraffin-embedded sections.

G. M. Allan*, J. A. Smyth, D. Todd, M. S. McNulty

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The development of a biotinylated in situ hybridization procedure for the detection of chicken anemia virus (CAV) is described. A double-stranded DNA probe was prepared using polymerase chain reaction and was biotinylated by nick translation. Hybridization conditions included the use of a microwave oven to denature target and probe nucleic acids and a five-step protocol for detection of biotinylated hybrids. In situ hybridization detected CAV nucleic acid in thymus tissue from experimentally infected birds after 6 hours, 3 days, and 7 days fixation time in formalin. However, immunocytochemical detection of CAV antigen was severely impaired if tissue was fixed in formalin for more than 6 hours.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalAvian Diseases
Volume37
Issue number1
DOIs
Publication statusPublished - 1993
Externally publishedYes

Bibliographical note

Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

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