In vitro activities of a novel antimicrobial peptide isolated from phyllomedusa tomopterna

Ziqi Chen, Xinping Xi, Yueyang Lu, Haiyan Hu, Ziyi Dong, Chengbang Ma, Lei Wang, Mei Zhou, Tianbao Chen, Shouying Du, Yang Lu

Research output: Contribution to journalArticlepeer-review

Abstract

Because of the abuse of antibiotics, clinical strains began to become more drug-resistant. Their evolution has long surpassed the speed of us looking for a new generation of antibacterial drugs. Therefore, it is urgent to discover a new antimicrobial substance to alleviate the pressure on conventional antibiotics. Antimicrobial peptides (AMP) are known for their significant activity towards a broad spectrum of bacteria, protozoa, yeasts, filamentous fungi. Here, we report a novel AMP named Dermaseptin-TO. Results demonstrate that Dermaseptin-TO can quickly exhibit antimicrobial activity to bacteria and yeast in a dose-related way. The highest minimum inhibit concentration (MIC) was observed in the E.faecalis group (128μM). Also, haemolytic outcomes showed no more than 10.65 % of red blood cells were affected when in the same concentrations or below. Besides, Dermaseptin‐TO also showed anticancer activity at a higher concentration. From the above, evidence proved that Phyllomedusine frog skin secretion is still a rich source that contains novel AMP and Dermaseptin-TO is competent to become an antimicrobial agent, its anticancer activity may broaden the way in basic cancer research. Also, following the same templates in molecular cloning may acquire new AMP classes with potent antimicrobial effects that could widen drug design in new anti-infective drugs.
Original languageEnglish
Article number104795
Number of pages10
JournalMicrobial Pathogenesis
Volume153
Early online date11 Feb 2021
DOIs
Publication statusPublished - Apr 2021

Keywords

  • Amphibian; Dermaseptins; Antimicrobial peptides; Anticancer peptides; Molecular cloning

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