In vitro activities of a novel antimicrobial peptide isolated from phyllomedusa tomopterna

Ziqi  Chen; Xinping Xi; Yueyang Lu; Haiyan  Hu; Ziyi  Dong; Chengbang Ma; Lei Wang; Mei Zhou; Tianbao Chen; Shouying  Du; Yang  Lu

doi:10.1016/j.micpath.2021.104795

In vitro activities of a novel antimicrobial peptide isolated from phyllomedusa tomopterna

Ziqi Chen, Xinping Xi, Yueyang Lu, Haiyan Hu, Ziyi Dong, Chengbang Ma, Lei Wang, Mei Zhou, Tianbao Chen, Shouying Du, Yang Lu

Research output: Contribution to journal › Article › peer-review

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Abstract

Because of the abuse of antibiotics, clinical strains began to become more drug-resistant. Their evolution has long surpassed the speed of us looking for a new generation of antibacterial drugs. Therefore, it is urgent to discover a new antimicrobial substance to alleviate the pressure on conventional antibiotics. Antimicrobial peptides (AMP) are known for their significant activity towards a broad spectrum of bacteria, protozoa, yeasts, filamentous fungi. Here, we report a novel AMP named Dermaseptin-TO. Results demonstrate that Dermaseptin-TO can quickly exhibit antimicrobial activity to bacteria and yeast in a dose-related way. The highest minimum inhibit concentration (MIC) was observed in the E.faecalis group (128μM). Also, haemolytic outcomes showed no more than 10.65 % of red blood cells were affected when in the same concentrations or below. Besides, Dermaseptin‐TO also showed anticancer activity at a higher concentration. From the above, evidence proved that Phyllomedusine frog skin secretion is still a rich source that contains novel AMP and Dermaseptin-TO is competent to become an antimicrobial agent, its anticancer activity may broaden the way in basic cancer research. Also, following the same templates in molecular cloning may acquire new AMP classes with potent antimicrobial effects that could widen drug design in new anti-infective drugs.

Original language	English
Article number	104795
Number of pages	10
Journal	Microbial Pathogenesis
Volume	153
Early online date	11 Feb 2021
DOIs	https://doi.org/10.1016/j.micpath.2021.104795
Publication status	Published - Apr 2021

Keywords

Amphibian; Dermaseptins; Antimicrobial peptides; Anticancer peptides; Molecular cloning

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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In vitro activities of a novel antimicrobial peptide isolated from phyllomedusa tomopterna
Copyright 2021 Elsevier. This manuscript is distributed under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.
Accepted author manuscript, 783 KBLicence: CC BY-NC-ND

Bioactive peptides from the skin secretion of Kassina senegalensis
Lu, Y. (Author), Zhou, M. (Supervisor), Chen, T. (Supervisor), Wang, L. (Supervisor) & Xi, X. (Supervisor), Dec 2021
Student thesis: Doctoral Thesis › Doctor of Philosophy

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title = "In vitro activities of a novel antimicrobial peptide isolated from phyllomedusa tomopterna",

abstract = "Because of the abuse of antibiotics, clinical strains began to become more drug-resistant. Their evolution has long surpassed the speed of us looking for a new generation of antibacterial drugs. Therefore, it is urgent to discover a new antimicrobial substance to alleviate the pressure on conventional antibiotics. Antimicrobial peptides (AMP) are known for their significant activity towards a broad spectrum of bacteria, protozoa, yeasts, filamentous fungi. Here, we report a novel AMP named Dermaseptin-TO. Results demonstrate that Dermaseptin-TO can quickly exhibit antimicrobial activity to bacteria and yeast in a dose-related way. The highest minimum inhibit concentration (MIC) was observed in the E.faecalis group (128μM). Also, haemolytic outcomes showed no more than 10.65 % of red blood cells were affected when in the same concentrations or below. Besides, Dermaseptin‐TO also showed anticancer activity at a higher concentration. From the above, evidence proved that Phyllomedusine frog skin secretion is still a rich source that contains novel AMP and Dermaseptin-TO is competent to become an antimicrobial agent, its anticancer activity may broaden the way in basic cancer research. Also, following the same templates in molecular cloning may acquire new AMP classes with potent antimicrobial effects that could widen drug design in new anti-infective drugs.",

keywords = "Amphibian; Dermaseptins; Antimicrobial peptides; Anticancer peptides; Molecular cloning",

author = "Ziqi Chen and Xinping Xi and Yueyang Lu and Haiyan Hu and Ziyi Dong and Chengbang Ma and Lei Wang and Mei Zhou and Tianbao Chen and Shouying Du and Yang Lu",

year = "2021",

month = apr,

doi = "10.1016/j.micpath.2021.104795",

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AU - Chen, Ziqi

AU - Xi, Xinping

AU - Lu, Yueyang

AU - Hu, Haiyan

AU - Dong, Ziyi

AU - Ma, Chengbang

AU - Wang, Lei

AU - Zhou, Mei

AU - Chen, Tianbao

AU - Du, Shouying

AU - Lu, Yang

PY - 2021/4

Y1 - 2021/4

N2 - Because of the abuse of antibiotics, clinical strains began to become more drug-resistant. Their evolution has long surpassed the speed of us looking for a new generation of antibacterial drugs. Therefore, it is urgent to discover a new antimicrobial substance to alleviate the pressure on conventional antibiotics. Antimicrobial peptides (AMP) are known for their significant activity towards a broad spectrum of bacteria, protozoa, yeasts, filamentous fungi. Here, we report a novel AMP named Dermaseptin-TO. Results demonstrate that Dermaseptin-TO can quickly exhibit antimicrobial activity to bacteria and yeast in a dose-related way. The highest minimum inhibit concentration (MIC) was observed in the E.faecalis group (128μM). Also, haemolytic outcomes showed no more than 10.65 % of red blood cells were affected when in the same concentrations or below. Besides, Dermaseptin‐TO also showed anticancer activity at a higher concentration. From the above, evidence proved that Phyllomedusine frog skin secretion is still a rich source that contains novel AMP and Dermaseptin-TO is competent to become an antimicrobial agent, its anticancer activity may broaden the way in basic cancer research. Also, following the same templates in molecular cloning may acquire new AMP classes with potent antimicrobial effects that could widen drug design in new anti-infective drugs.

AB - Because of the abuse of antibiotics, clinical strains began to become more drug-resistant. Their evolution has long surpassed the speed of us looking for a new generation of antibacterial drugs. Therefore, it is urgent to discover a new antimicrobial substance to alleviate the pressure on conventional antibiotics. Antimicrobial peptides (AMP) are known for their significant activity towards a broad spectrum of bacteria, protozoa, yeasts, filamentous fungi. Here, we report a novel AMP named Dermaseptin-TO. Results demonstrate that Dermaseptin-TO can quickly exhibit antimicrobial activity to bacteria and yeast in a dose-related way. The highest minimum inhibit concentration (MIC) was observed in the E.faecalis group (128μM). Also, haemolytic outcomes showed no more than 10.65 % of red blood cells were affected when in the same concentrations or below. Besides, Dermaseptin‐TO also showed anticancer activity at a higher concentration. From the above, evidence proved that Phyllomedusine frog skin secretion is still a rich source that contains novel AMP and Dermaseptin-TO is competent to become an antimicrobial agent, its anticancer activity may broaden the way in basic cancer research. Also, following the same templates in molecular cloning may acquire new AMP classes with potent antimicrobial effects that could widen drug design in new anti-infective drugs.

KW - Amphibian; Dermaseptins; Antimicrobial peptides; Anticancer peptides; Molecular cloning

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DO - 10.1016/j.micpath.2021.104795

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JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

M1 - 104795

ER -

In vitro activities of a novel antimicrobial peptide isolated from phyllomedusa tomopterna

Abstract

Keywords

UN SDGs

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Student theses

Bioactive peptides from the skin secretion of Kassina senegalensis

Cite this