TY - CHAP
T1 - In vitro and in vivo methodologies for studying the Sigma 54-dependent transcription.
AU - Buck, Martin
AU - Engl, Christoph
AU - Joly, Nicolas
AU - Jovanovic, Goran
AU - Lawton, Edward
AU - McDonald, Christopher
AU - Schumacher, Jorg
AU - Waite, Christopher J.
AU - Zhang, Nan
PY - 2015/1/28
Y1 - 2015/1/28
N2 - Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ54), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ54-dependent gene expression.
AB - Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ54), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ54-dependent gene expression.
U2 - 10.1007/978-1-4939-2392-2_4
DO - 10.1007/978-1-4939-2392-2_4
M3 - Chapter (peer-reviewed)
SN - 9781493923915
VL - 1276
T3 - Methods in Molecular Biology
SP - 53
EP - 79
BT - Bacterial Transcriptional Control: methods and protocols
A2 - Artsimovitch, Irina
A2 - Santangelo, Thomas J
PB - Springer
CY - New York
ER -