In vitro - in vivo relations for the parenteral liposomal formulation of Amphotericin B: A clinically relevant approach with PBPK modeling

Ricardo Díaz de León–Ortega, Deirdre D'Arcy, Dimitrios Lamprou, Nikoletta Fotaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In vitro release testing is a useful tool for the quality control of controlled release parenteral formulations, but in vitro release test conditions that reflect or are able to predict the in vivo performance are advantageous. Therefore, it is important to investigate the factors that could affect drug release from formulations and relate them to in vivo performance. In this study the effect of media composition including albumin presence, type of buffer and hydrodynamics on drug release were evaluated on a liposomal Amphotericin B formulation (Ambisome®). A physiologically based pharmacokinetic (PBPK) model was developed using plasma concentration profiles from healthy subjects, in order to investigate the impact of each variable from the in vitro release tests on the prediction of the in vivo performance. It was found that albumin presence was the most important factor for the release of Amphotericin B from Ambisome®; both hydrodynamics setups, coupled with the PBPK model, had comparable predictive ability for simulating in vivo plasma concentration profiles. The PBPK model was extrapolated to a hypothetical hypoalbuminaemic population and the Amphotericin B plasma concentration and its activity against fungal cells were simulated. Selected in vitro release tests for these controlled release parenteral formulations were able to predict the in vivo AmB exposure, and this PBPK driven approach to release test development could benefit development of such formulations.
Original languageEnglish
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Early online date05 Mar 2020
DOIs
Publication statusEarly online date - 05 Mar 2020

Keywords

  • Amphotericin B
  • Liposomes
  • PBPK
  • PBPKPD
  • Release
  • in vitro
  • modeling

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