IN VITRO INFLAMMATORY RESPONSE EVALUATION OF PRE-DEGRADED BIORESORBABLE POLYMERS USED IN TRAUMA FIXATION AND TISSUE REGENERATION APPLICATIONS

Research output: Contribution to conferenceAbstract

Abstract

Research in orthopaedics is now moving away from permanent metallic implants, and looking towards the use of bioresorbable polymers (e.g. PLLA, PGA and related co-polymers) that, when implanted into the injured site, bioresorb as the tissue heals. However, reports of a delayed inflammatory reponse occurring in the late stages of polymer degradation has limited the wide scale use of these polymers. Few studies assess the long-term biocompatibility of these polymers and with an increasing market for bioresorbable materials it is anticipated that this will be a future issue.
This work aims to develop a predictive tool that can be used to assess the delayed inflammatory response of poly(D,L-lactide-co-glycolide) (PDLGA) using in vitro tests. An elevated temperature accelerated test (47oC) was developed and utilitised to induce predetermined amounts of degradation in PDLGA. This was used to mimic a range of clinically relevant in vivo implantation times up to ?? weeks/months. All pre-degradion work was performed under sterile conditions, in PBS solution. At predetermined time intervals, indicators of late stage inflammation will be assessed using an MTT cytotoxicity assay, an inflammation antibody array and an ELISA analysis for inflammatory factors, with mouse L929 fibroblasts. It is hypothosised that at the later degradation time intervals signs of inflammatory factors will be observed.
LanguageEnglish
Publication statusPublished - 26 Sep 2018
Event26th Annual Meeting of European Orthopaedic Research Society - The Galmont Hotel, Galway, Ireland
Duration: 25 Sep 201828 Sep 2018
http://www.eors2018.org/

Conference

Conference26th Annual Meeting of European Orthopaedic Research Society
Abbreviated titleEORS 2018
CountryIreland
CityGalway
Period25/09/201828/09/2018
Internet address

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Tissue Fixation
Regeneration
Polymers
Wounds and Injuries
Prostaglandins A
Inflammation
Statistical Factor Analysis
Orthopedics
Fibroblasts
Enzyme-Linked Immunosorbent Assay
Temperature
In Vitro Techniques
Antibodies
Research

Cite this

@conference{d9d7e7a85d0640d697eb5d2005c95b5a,
title = "IN VITRO INFLAMMATORY RESPONSE EVALUATION OF PRE-DEGRADED BIORESORBABLE POLYMERS USED IN TRAUMA FIXATION AND TISSUE REGENERATION APPLICATIONS",
abstract = "Research in orthopaedics is now moving away from permanent metallic implants, and looking towards the use of bioresorbable polymers (e.g. PLLA, PGA and related co-polymers) that, when implanted into the injured site, bioresorb as the tissue heals. However, reports of a delayed inflammatory reponse occurring in the late stages of polymer degradation has limited the wide scale use of these polymers. Few studies assess the long-term biocompatibility of these polymers and with an increasing market for bioresorbable materials it is anticipated that this will be a future issue. This work aims to develop a predictive tool that can be used to assess the delayed inflammatory response of poly(D,L-lactide-co-glycolide) (PDLGA) using in vitro tests. An elevated temperature accelerated test (47oC) was developed and utilitised to induce predetermined amounts of degradation in PDLGA. This was used to mimic a range of clinically relevant in vivo implantation times up to ?? weeks/months. All pre-degradion work was performed under sterile conditions, in PBS solution. At predetermined time intervals, indicators of late stage inflammation will be assessed using an MTT cytotoxicity assay, an inflammation antibody array and an ELISA analysis for inflammatory factors, with mouse L929 fibroblasts. It is hypothosised that at the later degradation time intervals signs of inflammatory factors will be observed.",
author = "Lucy Geddes and Louise Carson and Efrosyni Themistou and Fraser Buchanan",
year = "2018",
month = "9",
day = "26",
language = "English",
note = "26th Annual Meeting of European Orthopaedic Research Society, EORS 2018 ; Conference date: 25-09-2018 Through 28-09-2018",
url = "http://www.eors2018.org/",

}

IN VITRO INFLAMMATORY RESPONSE EVALUATION OF PRE-DEGRADED BIORESORBABLE POLYMERS USED IN TRAUMA FIXATION AND TISSUE REGENERATION APPLICATIONS. / Geddes, Lucy; Carson, Louise; Themistou, Efrosyni; Buchanan, Fraser.

2018. Abstract from 26th Annual Meeting of European Orthopaedic Research Society, Galway, Ireland.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - IN VITRO INFLAMMATORY RESPONSE EVALUATION OF PRE-DEGRADED BIORESORBABLE POLYMERS USED IN TRAUMA FIXATION AND TISSUE REGENERATION APPLICATIONS

AU - Geddes, Lucy

AU - Carson, Louise

AU - Themistou, Efrosyni

AU - Buchanan, Fraser

PY - 2018/9/26

Y1 - 2018/9/26

N2 - Research in orthopaedics is now moving away from permanent metallic implants, and looking towards the use of bioresorbable polymers (e.g. PLLA, PGA and related co-polymers) that, when implanted into the injured site, bioresorb as the tissue heals. However, reports of a delayed inflammatory reponse occurring in the late stages of polymer degradation has limited the wide scale use of these polymers. Few studies assess the long-term biocompatibility of these polymers and with an increasing market for bioresorbable materials it is anticipated that this will be a future issue. This work aims to develop a predictive tool that can be used to assess the delayed inflammatory response of poly(D,L-lactide-co-glycolide) (PDLGA) using in vitro tests. An elevated temperature accelerated test (47oC) was developed and utilitised to induce predetermined amounts of degradation in PDLGA. This was used to mimic a range of clinically relevant in vivo implantation times up to ?? weeks/months. All pre-degradion work was performed under sterile conditions, in PBS solution. At predetermined time intervals, indicators of late stage inflammation will be assessed using an MTT cytotoxicity assay, an inflammation antibody array and an ELISA analysis for inflammatory factors, with mouse L929 fibroblasts. It is hypothosised that at the later degradation time intervals signs of inflammatory factors will be observed.

AB - Research in orthopaedics is now moving away from permanent metallic implants, and looking towards the use of bioresorbable polymers (e.g. PLLA, PGA and related co-polymers) that, when implanted into the injured site, bioresorb as the tissue heals. However, reports of a delayed inflammatory reponse occurring in the late stages of polymer degradation has limited the wide scale use of these polymers. Few studies assess the long-term biocompatibility of these polymers and with an increasing market for bioresorbable materials it is anticipated that this will be a future issue. This work aims to develop a predictive tool that can be used to assess the delayed inflammatory response of poly(D,L-lactide-co-glycolide) (PDLGA) using in vitro tests. An elevated temperature accelerated test (47oC) was developed and utilitised to induce predetermined amounts of degradation in PDLGA. This was used to mimic a range of clinically relevant in vivo implantation times up to ?? weeks/months. All pre-degradion work was performed under sterile conditions, in PBS solution. At predetermined time intervals, indicators of late stage inflammation will be assessed using an MTT cytotoxicity assay, an inflammation antibody array and an ELISA analysis for inflammatory factors, with mouse L929 fibroblasts. It is hypothosised that at the later degradation time intervals signs of inflammatory factors will be observed.

UR - http://www.eors2018.org/

M3 - Abstract

ER -