In vitro o-antigen ligase assay

Xiang Ruan; Miguel A Valvano

doi:10.1007/978-1-62703-465-4_15

In vitro o-antigen ligase assay

Xiang Ruan, Miguel A Valvano

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.

Original language	English
Pages (from-to)	185-97
Journal	Methods in Molecular Biology
Volume	1022
DOIs	https://doi.org/10.1007/978-1-62703-465-4_15
Publication status	Published - 16 May 2013

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title = "In vitro o-antigen ligase assay",

abstract = "WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.",

author = "Xiang Ruan and Valvano, {Miguel A}",

year = "2013",

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T1 - In vitro o-antigen ligase assay

AU - Ruan, Xiang

AU - Valvano, Miguel A

PY - 2013/5/16

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N2 - WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.

AB - WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.

U2 - 10.1007/978-1-62703-465-4_15

DO - 10.1007/978-1-62703-465-4_15

M3 - Article

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SN - 1940-6029

VL - 1022

SP - 185

EP - 197

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

In vitro o-antigen ligase assay

Abstract

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