In vitro o-antigen ligase assay

Xiang Ruan, Miguel A Valvano

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.
Original languageEnglish
Pages (from-to)185-97
JournalMethods in Molecular Biology
Publication statusPublished - 16 May 2013


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