In vitro viability of choroidal melanomata following pre-enucleation irradiation

U. Chakravarthy; L. Frew; T. A. Gardiner; D. B. Archer

In vitro viability of choroidal melanomata following pre-enucleation irradiation

U. Chakravarthy, L. Frew, T. A. Gardiner, D. B. Archer

Research output: Contribution to journal › Article › peer-review

Abstract

In a group of eighteen patients with uveal melanomas, seven underwent low-dose pre-enucleation irradiation of approximately 2000 cGy. All the tumours were propagated in tissue culture and the growth characteristics of tumour cells from irradiated eyes were compared with tumour cells from non-irradiated eyes. Cultures were observed with phase-contrast microscopy, and radioactive thymidine labelling was used to study cell turnover. Although tissue samples from peripheral areas of irradiated tumours produced a mixture of viable and non-viable cells, with reduced ability to attach to substrate, central regions of irradiated tumours contained viable cells which propagated freely in tissue culture.

Original language	Undefined/Unknown
Pages (from-to)	303-310
Number of pages	8
Journal	International Ophthalmology
Volume	15
Issue number	5
Publication status	Published - 01 Sep 1991

Bibliographical note

LR: 20061115; JID: 7904294; 0 (Cobalt Radioisotopes); ppublish

Keywords

Cell Differentiation/radiation effects
Cell Division/radiation effects
Cell Survival/radiation effects
Choroid Neoplasms/pathology/radiotherapy/surgery
Cobalt Radioisotopes
Culture Techniques
Eye Enucleation
Humans
Melanoma/pathology/radiotherapy/surgery
Microscopy, Phase-Contrast
Radiation Dosage
Tumor Cells, Cultured

Cite this

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title = "In vitro viability of choroidal melanomata following pre-enucleation irradiation",

abstract = "In a group of eighteen patients with uveal melanomas, seven underwent low-dose pre-enucleation irradiation of approximately 2000 cGy. All the tumours were propagated in tissue culture and the growth characteristics of tumour cells from irradiated eyes were compared with tumour cells from non-irradiated eyes. Cultures were observed with phase-contrast microscopy, and radioactive thymidine labelling was used to study cell turnover. Although tissue samples from peripheral areas of irradiated tumours produced a mixture of viable and non-viable cells, with reduced ability to attach to substrate, central regions of irradiated tumours contained viable cells which propagated freely in tissue culture.",

keywords = "Cell Differentiation/radiation effects, Cell Division/radiation effects, Cell Survival/radiation effects, Choroid Neoplasms/pathology/radiotherapy/surgery, Cobalt Radioisotopes, Culture Techniques, Eye Enucleation, Humans, Melanoma/pathology/radiotherapy/surgery, Microscopy, Phase-Contrast, Radiation Dosage, Tumor Cells, Cultured",

author = "U. Chakravarthy and L. Frew and Gardiner, {T. A.} and Archer, {D. B.}",

note = "LR: 20061115; JID: 7904294; 0 (Cobalt Radioisotopes); ppublish",

year = "1991",

month = sep,

day = "1",

language = "Undefined/Unknown",

volume = "15",

pages = "303--310",

journal = "International Ophthalmology",

issn = "0165-5701",

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}

TY - JOUR

T1 - In vitro viability of choroidal melanomata following pre-enucleation irradiation

AU - Chakravarthy, U.

AU - Frew, L.

AU - Gardiner, T. A.

AU - Archer, D. B.

N1 - LR: 20061115; JID: 7904294; 0 (Cobalt Radioisotopes); ppublish

PY - 1991/9/1

Y1 - 1991/9/1

N2 - In a group of eighteen patients with uveal melanomas, seven underwent low-dose pre-enucleation irradiation of approximately 2000 cGy. All the tumours were propagated in tissue culture and the growth characteristics of tumour cells from irradiated eyes were compared with tumour cells from non-irradiated eyes. Cultures were observed with phase-contrast microscopy, and radioactive thymidine labelling was used to study cell turnover. Although tissue samples from peripheral areas of irradiated tumours produced a mixture of viable and non-viable cells, with reduced ability to attach to substrate, central regions of irradiated tumours contained viable cells which propagated freely in tissue culture.

AB - In a group of eighteen patients with uveal melanomas, seven underwent low-dose pre-enucleation irradiation of approximately 2000 cGy. All the tumours were propagated in tissue culture and the growth characteristics of tumour cells from irradiated eyes were compared with tumour cells from non-irradiated eyes. Cultures were observed with phase-contrast microscopy, and radioactive thymidine labelling was used to study cell turnover. Although tissue samples from peripheral areas of irradiated tumours produced a mixture of viable and non-viable cells, with reduced ability to attach to substrate, central regions of irradiated tumours contained viable cells which propagated freely in tissue culture.

KW - Cell Differentiation/radiation effects

KW - Cell Division/radiation effects

KW - Cell Survival/radiation effects

KW - Choroid Neoplasms/pathology/radiotherapy/surgery

KW - Cobalt Radioisotopes

KW - Culture Techniques

KW - Eye Enucleation

KW - Humans

KW - Melanoma/pathology/radiotherapy/surgery

KW - Microscopy, Phase-Contrast

KW - Radiation Dosage

KW - Tumor Cells, Cultured

M3 - Article

VL - 15

SP - 303

EP - 310

JO - International Ophthalmology

JF - International Ophthalmology

SN - 0165-5701

IS - 5

ER -