In vitro viability of choroidal melanomata following pre-enucleation irradiation

U. Chakravarthy, L. Frew, T. A. Gardiner, D. B. Archer

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In a group of eighteen patients with uveal melanomas, seven underwent low-dose pre-enucleation irradiation of approximately 2000 cGy. All the tumours were propagated in tissue culture and the growth characteristics of tumour cells from irradiated eyes were compared with tumour cells from non-irradiated eyes. Cultures were observed with phase-contrast microscopy, and radioactive thymidine labelling was used to study cell turnover. Although tissue samples from peripheral areas of irradiated tumours produced a mixture of viable and non-viable cells, with reduced ability to attach to substrate, central regions of irradiated tumours contained viable cells which propagated freely in tissue culture.
Original languageUndefined/Unknown
Pages (from-to)303-310
Number of pages8
JournalInternational ophthalmology
Volume15
Issue number5
Publication statusPublished - 01 Sep 1991

Bibliographical note

LR: 20061115; JID: 7904294; 0 (Cobalt Radioisotopes); ppublish

Keywords

  • Cell Differentiation/radiation effects
  • Cell Division/radiation effects
  • Cell Survival/radiation effects
  • Choroid Neoplasms/pathology/radiotherapy/surgery
  • Cobalt Radioisotopes
  • Culture Techniques
  • Eye Enucleation
  • Humans
  • Melanoma/pathology/radiotherapy/surgery
  • Microscopy, Phase-Contrast
  • Radiation Dosage
  • Tumor Cells, Cultured

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