Incomplete DJH rearrangements.

D. González, R. Garcia-Sanz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Analysis of Ig genes in B-cell malignancies has become an essential method in molecular diagnosis, and polymerase chain reaction (PCR) amplification of Ig heavy chain gene (IgH) rearrangements is now widely used for detection of clonality and minimal residual disease (MRD). Although several different sensitive protocols are now available for PCR analysis of IgH genes, they are frequently hampered owing to the high rate of somatic hypermutation present in multiple myeloma (MM). We recently described a new approach using incomplete DJH rearrangements as an alternative target. About 60% of MM samples contain an incomplete DJH rearrangement, 90% of them lacking on somatic mutations. This approach allows resolution of problems derived from primer mismatches, making DJH rearrangement a reliable and sensitive target for detection of clonality and MRD investigation in MM.
Original languageEnglish
Pages (from-to)165-173
Number of pages9
JournalMethods in molecular medicine
Volume113
DOIs
Publication statusPublished - 2005

Keywords

  • B-Lymphocytes
  • Base Sequence
  • Gene Rearrangement
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain
  • Humans
  • Multiple Myeloma
  • Mutation
  • Polymerase Chain Reaction

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