Increased expression of fibroblast activation protein-alpha in keloid fibroblasts: implications for development of a novel treatment option.

K. Dienus, A. Bayat, Brendan Gilmore, Oliver Seifert

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Keloid scars are common benign fibroproliferative reticular dermal lesions with unknown etiology and ill-defined management with high rate of recurrence post surgery. The progression of keloids is characterized by increased deposition of extracellular matrix proteins, invasion into the surrounding healthy skin and inflammation. Fibroblasts are considered to be the key cellular mediators of fibrogenesis in keloid scars. Fibroblast activation protein alpha (FAP-a) and dipeptidyl peptidase IV (DPPIV) are proteases located at the plasma membrane promoting cell invasiveness and tumor growth and have been previously associated with keloid scars. Therefore, in this study we analyzed in further detail the expression of FAP-a in keloid fibroblasts compared to control skin fibroblasts. Dermal fibroblasts were obtained from punch-biopsies from the active margin of four keloids and four control skin samples. Flow cytometry was used to analyze FAP-a expression and the CytoSelect(®) 24-Well Collagen I Cell Invasion Assay was applied to study fibroblast invasion. Secretion of extracellular matrix (ECM) proteins was investigated by multiplexed particle-based flow cytometric assay and enzyme-linked immunosorbent assay. We found an increased expression of FAP-a in keloid fibroblasts compared to control skin fibroblasts (p
Original languageEnglish
Pages (from-to)725-731
Number of pages7
JournalArchives of Dermatological Research
VolumeAhead of Print
Issue number10
DOIs
Publication statusPublished - Dec 2010

ASJC Scopus subject areas

  • Dermatology

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