Influence of dietary oils rich in omega-6 or omega-3 fatty acids on rumen microbiome of dairy cows

Nathaly Cancino-Padilla, Felipe Gajardo, André Luis Alves Neves, Ahmed Eid Kholif, Marcello Mele, Sharon A Huws, Juan J Loor, Jaime Romero, Einar Vargas-Bello-Pérez

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
5 Downloads (Pure)


The objective of this study was to compare the effect of supplementing dairy cow diets with contrasting sources of omega-6 (soybean oil) and omega-3 (fish oil) PUFA on rumen microbiome. For 63 days, 15 mid-lactating cows were fed a control diet (n = 5 cows; no fat supplement) or control diet supplemented with 2.9% DM of either soybean oil (SO; n = 5 cows) or fish oil (FO; n = 5 cows). Ruminal contents were collected on days 0, 21, 42 and 63 for 16S rRNA gene sequencing. Beta diversity and Shannon, Simpson and Chao1 diversity indices were not affected by dietary treatments. In terms of core microbiome, Succiniclasticum, Prevotella, Rikenellaceae_RC9_gut_group and NK4A214_group were the most prevalent taxa regardless of treatments. Bifidobacterium was absent in SO diet, Acetitomaculum was absent in FO, and Sharpea, was only detected in SO. Overall, results showed that at 2.9% DM supplementation of either SO or FO over 63 days in dairy cow diets does not cause major impact on bacterial community composition and thus is recommended as feeding practice.
Original languageEnglish
Article numbertxad074
Number of pages10
JournalTranslational Animal Science
Issue number1
Early online date01 Jul 2023
Publication statusPublished - 2023
Externally publishedYes


  • General Veterinary
  • Animal Science and Zoology


Dive into the research topics of 'Influence of dietary oils rich in omega-6 or omega-3 fatty acids on rumen microbiome of dairy cows'. Together they form a unique fingerprint.

Cite this