The N-terminal propeptide domains of several cathepsin L-like cysteine proteases have been shown to possess potent inhibitory activity. Here we report the first kinetic characterisation of the inhibition properties of the cathepsin V propeptide (CatV PP). Using a facile recombinant approach we demonstrate expression, purification and evaluation of the CatV PP. This propeptide was found to behave as a tight-binding inhibitor against CatV (K (i) 10.2 nm). It also functions as an inhibitor against other members of the CatL-like subclass (CatL, 9.8 nm; CatS, 10.7 nm; and CatK, 149 nm) and had no discernible effects upon the more distantly related CatB.
Burden, R., Snoddy, P., Jefferies, C. A., Walker, B., & Scott, C. (2007). Inhibition of cathepsin L-like proteases by cathepsin V propeptide. Biological Chemistry, 388(5)(5), 541-545. https://doi.org/10.1515/BC.2007.053