Inhibition of cathepsin L-like proteases by cathepsin V propeptide

Roberta Burden, P. Snoddy, C.A. Jefferies, Brian Walker, Christopher Scott

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The N-terminal propeptide domains of several cathepsin L-like cysteine proteases have been shown to possess potent inhibitory activity. Here we report the first kinetic characterisation of the inhibition properties of the cathepsin V propeptide (CatV PP). Using a facile recombinant approach we demonstrate expression, purification and evaluation of the CatV PP. This propeptide was found to behave as a tight-binding inhibitor against CatV (K (i) 10.2 nm). It also functions as an inhibitor against other members of the CatL-like subclass (CatL, 9.8 nm; CatS, 10.7 nm; and CatK, 149 nm) and had no discernible effects upon the more distantly related CatB.
Original languageEnglish
Pages (from-to)541-545
Number of pages5
JournalBiological Chemistry
Volume388(5)
Issue number5
DOIs
Publication statusPublished - 01 May 2007

ASJC Scopus subject areas

  • Biochemistry

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