Intensified crude glycerol conversion to butanol by immobilized Clostridium pasteurianum

Vladimír Krasňan, Michal Plž, Andrew C. Marr, Kristína Markošová, Michal Rosenberg, Martin Rebros

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)
372 Downloads (Pure)


Butanol production from glycerol was investigated through Clostridium pasteurianum entrapped into polyvinyl alcohol particles. Using an optimized system, batch and repeated batch fermentations with free and entrapped cells were performed, respectively. In both systems, glycerol samples of different purity were tested. In repeated batch fermentations, process time decreased from 19.5 to 2.7 hours and butanol productivity increased 6.3 times (3.08 g.L-1.h-1) compared with the free-cell process, using pure glycerol. In the case of glycerol from biodiesel production, butanol productivity of 2.90 and 1.76 g.L-1.h-1 was achieved from glycerol 01 and glycerol 02, respectively. No cell growth or solvent production was observed when the same glycerols were used in free-cell fermentations.
Original languageEnglish
JournalBiochemical Engineering Journal
Early online date11 Mar 2018
Publication statusEarly online date - 11 Mar 2018


Dive into the research topics of 'Intensified crude glycerol conversion to butanol by immobilized Clostridium pasteurianum'. Together they form a unique fingerprint.

Cite this