Interaction of preimplantation factor with the global bovine endometrial transcriptome

Ruth E. Wonfor*, Christopher J. Creevey, Manuela Natoli, Matthew Hegarty, Deborah M. Nash, Michael T. Rose

*Corresponding author for this work

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Abstract

Preimplantation factor (PIF) is an embryo derived peptide which exerts an immune modulatory effect on human endometrium, promoting immune tolerance to the embryo whilst maintaining the immune response to invading pathogens. While bovine embryos secrete PIF, the effect on the bovine endometrium is unknown. Maternal recognition of pregnancy is driven by an embryo-maternal cross talk, however the process differs between humans and cattle. As many embryos are lost during the early part of pregnancy in cattle, a greater knowledge of factors affecting the embryo-maternal crosstalk, such as PIF, is needed to improve fertility. Therefore, for the first time, we demonstrate the effect of synthetic PIF (sPIF) on the bovine transcriptome in an ex vivo bovine endometrial tissue culture model. Explants were cultured for 30h with sPIF (100nM) or in control media. Total RNA was analysed via RNA-sequencing. As a result of sPIF treatment, 102 genes were differentially expressed compared to the control (Padj<0.1), although none by more than 2-fold. The majority of genes (78) were downregulated. Pathway analysis revealed targeting of several immune based pathways. Genes for the TNF, NF-κB, IL-17, MAPK and TLR signalling pathways were down-regulated by sPIF. However, some immune genes were demonstrated to be upregulated following sPIF treatment, including C3. Steroid biosynthesis was the only over-represented pathway with all genes upregulated. We demonstrate that sPIF can modulate the bovine endometrial transcriptome in an immune modulatory manner, like that in the human endometrium, however, the regulation of genes was much weaker than in previous human work.

Original languageEnglish
Article numbere0242874
Number of pages21
JournalPLoS ONE
Volume15
Issue number12
DOIs
Publication statusPublished - 07 Dec 2020

Bibliographical note

Funding Information:
R. Wonfor was funded by Aberystwyth University through the Doctoral Career Development Scheme. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors extend their thanks to Dr Eytan Barnea, BioIncept LLC (New Jersey, USA) for the kind donation of sPIF, Dr Colin Sauze for bioinformatics technical support and the staff at Randall Parker Foods for assistance in sample collection.

Publisher Copyright:
© 2020 Wonfor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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