Interactions of a non-thermal atmospheric pressure plasma effluent with PC-3 prostate cancer cells

Andrew Gibson; Helen O. McCarthy; Ahlam Ali; Deborah O'Connell; William G. Graham

doi:10.1002/ppap.201400111

Interactions of a non-thermal atmospheric pressure plasma effluent with PC-3 prostate cancer cells

Andrew Gibson, Helen O. McCarthy, Ahlam Ali, Deborah O'Connell, William G. Graham

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

The effect of a radio-frequency driven, microscale non thermal atmospheric pressure plasma jet operated in helium with vol. 0.3% molecular oxygen gas admixture, on PC-3 prostate cancer cells has been investigated. The viability of cells exposed to the plasma was found to decrease with increasing plasma exposure time, with apoptosis through caspase and PARP cleavage being observed. High concentrations of nitrite and nitrate were detected in growth media exposed to the plasma and were found to increase in a time dependent manner post exposure. This indicates a slow release of reactive nitrogen species into the growth media, which is likely to influence cellular response to plasma exposure.

Original language	English
Pages (from-to)	1142-1149
Number of pages	8
Journal	Plasma Processes and Polymers
Volume	11
Issue number	12
Early online date	12 Sept 2014
DOIs	https://doi.org/10.1002/ppap.201400111
Publication status	Published - Dec 2014

Keywords

Apoptosis
Cancer
Nitric oxide
Plasma jet
Radicals

ASJC Scopus subject areas

Condensed Matter Physics
Polymers and Plastics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/ppap.201400111

Cite this

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abstract = "The effect of a radio-frequency driven, microscale non thermal atmospheric pressure plasma jet operated in helium with vol. 0.3% molecular oxygen gas admixture, on PC-3 prostate cancer cells has been investigated. The viability of cells exposed to the plasma was found to decrease with increasing plasma exposure time, with apoptosis through caspase and PARP cleavage being observed. High concentrations of nitrite and nitrate were detected in growth media exposed to the plasma and were found to increase in a time dependent manner post exposure. This indicates a slow release of reactive nitrogen species into the growth media, which is likely to influence cellular response to plasma exposure.",

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AU - McCarthy, Helen O.

AU - Ali, Ahlam

AU - O'Connell, Deborah

AU - Graham, William G.

PY - 2014/12

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N2 - The effect of a radio-frequency driven, microscale non thermal atmospheric pressure plasma jet operated in helium with vol. 0.3% molecular oxygen gas admixture, on PC-3 prostate cancer cells has been investigated. The viability of cells exposed to the plasma was found to decrease with increasing plasma exposure time, with apoptosis through caspase and PARP cleavage being observed. High concentrations of nitrite and nitrate were detected in growth media exposed to the plasma and were found to increase in a time dependent manner post exposure. This indicates a slow release of reactive nitrogen species into the growth media, which is likely to influence cellular response to plasma exposure.

AB - The effect of a radio-frequency driven, microscale non thermal atmospheric pressure plasma jet operated in helium with vol. 0.3% molecular oxygen gas admixture, on PC-3 prostate cancer cells has been investigated. The viability of cells exposed to the plasma was found to decrease with increasing plasma exposure time, with apoptosis through caspase and PARP cleavage being observed. High concentrations of nitrite and nitrate were detected in growth media exposed to the plasma and were found to increase in a time dependent manner post exposure. This indicates a slow release of reactive nitrogen species into the growth media, which is likely to influence cellular response to plasma exposure.

KW - Apoptosis

KW - Cancer

KW - Nitric oxide

KW - Plasma jet

KW - Radicals

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ER -

Interactions of a non-thermal atmospheric pressure plasma effluent with PC-3 prostate cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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