Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

G. M. Allan; F. Mc Neilly; B. M. Meehan; S. Kennedy; D. P. MacKie; J. A. Ellis; E. G. Clark; E. Espuna; N. Saubi; P. Riera; A. Bøtner; C. E. Charreyre

doi:10.1016/S0378-1135(99)00004-8

Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

G. M. Allan^*, F. Mc Neilly, B. M. Meehan, S. Kennedy, D. P. MacKie, J. A. Ellis, E. G. Clark, E. Espuna, N. Saubi, P. Riera, A. Bøtner, C. E. Charreyre

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

177 Citations (Scopus)

Abstract

A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1). Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	115-123
Number of pages	9
Journal	Veterinary Microbiology
Volume	66
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1135(99)00004-8
Publication status	Published - 01 Apr 1999
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Merial S.A.S. for financial support. The authors are grateful to Mr. Cliff Mason for the production of photographs. The authors would like to acknowledge the use of the Seqnet facility at the Daresbury Laboratory, Warrington, UK.

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

Genotyping
Polymerase chain reaction
Porcine circovirus
Wasting syndromes

ASJC Scopus subject areas

Microbiology
General Veterinary

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10.1016/S0378-1135(99)00004-8

Cite this

Allan, G. M., Mc Neilly, F., Meehan, B. M., Kennedy, S., MacKie, D. P., Ellis, J. A., Clark, E. G., Espuna, E., Saubi, N., Riera, P., Bøtner, A., & Charreyre, C. E. (1999). Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Veterinary Microbiology, 66(2), 115-123. https://doi.org/10.1016/S0378-1135(99)00004-8

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abstract = "A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1). Copyright (C) 1999 Elsevier Science B.V.",

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author = "Allan, {G. M.} and {Mc Neilly}, F. and Meehan, {B. M.} and S. Kennedy and MacKie, {D. P.} and Ellis, {J. A.} and Clark, {E. G.} and E. Espuna and N. Saubi and P. Riera and A. B{\o}tner and Charreyre, {C. E.}",

note = "Funding Information: We would like to thank Merial S.A.S. for financial support. The authors are grateful to Mr. Cliff Mason for the production of photographs. The authors would like to acknowledge the use of the Seqnet facility at the Daresbury Laboratory, Warrington, UK. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

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AU - Meehan, B. M.

AU - Kennedy, S.

AU - MacKie, D. P.

AU - Ellis, J. A.

AU - Clark, E. G.

AU - Espuna, E.

AU - Saubi, N.

AU - Riera, P.

AU - Bøtner, A.

AU - Charreyre, C. E.

N1 - Funding Information: We would like to thank Merial S.A.S. for financial support. The authors are grateful to Mr. Cliff Mason for the production of photographs. The authors would like to acknowledge the use of the Seqnet facility at the Daresbury Laboratory, Warrington, UK. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

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N2 - A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1). Copyright (C) 1999 Elsevier Science B.V.

AB - A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1). Copyright (C) 1999 Elsevier Science B.V.

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Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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