Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland

John McKillen; F. McNeilly; Catherine Duffy; Michael McMenamy; I. McNair; Bernt Hjertner; Andrena Millar; K. Mckay; P. Lagan; Brian Adair; Gordon Allan

doi:10.1016/j.vetmic.2011.04.013

Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland

John McKillen, F. McNeilly, Catherine Duffy, Michael McMenamy, I. McNair, Bernt Hjertner, Andrena Millar, K. Mckay, P. Lagan, Brian Adair, Gordon Allan

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

We report the isolation in cell cultures of two novel bocavirus species in pigs from farms in Northern Ireland with clinical postweaning multisystemic wasting syndrome (PMWS). We have designated the isolates as porcine bocavirus-3 (PBoV3) and porcine bocavirus-4 (PBoV4). To date 5082 and 4125 bps of PBoV3 and PBoV4 have been sequenced, respectively. PBoV3 and PBoV4 show nucleotide homology to other known bocaviruses in swine and other organisms. Open reading frame (ORF) analysis has shown that these viruses have a third small ORF, equivalent to the NP1 ORF that distinguishes the bocaviruses from other parvoviruses.

Original language	English
Pages (from-to)	39-45
Number of pages	7
Journal	Veterinary Microbiology
Volume	152
Issue number	1-2
DOIs	https://doi.org/10.1016/j.vetmic.2011.04.013
Publication status	Published - 26 Aug 2011

ASJC Scopus subject areas

Microbiology
General Veterinary

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title = "Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland",

abstract = "We report the isolation in cell cultures of two novel bocavirus species in pigs from farms in Northern Ireland with clinical postweaning multisystemic wasting syndrome (PMWS). We have designated the isolates as porcine bocavirus-3 (PBoV3) and porcine bocavirus-4 (PBoV4). To date 5082 and 4125 bps of PBoV3 and PBoV4 have been sequenced, respectively. PBoV3 and PBoV4 show nucleotide homology to other known bocaviruses in swine and other organisms. Open reading frame (ORF) analysis has shown that these viruses have a third small ORF, equivalent to the NP1 ORF that distinguishes the bocaviruses from other parvoviruses.",

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T1 - Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland

AU - McKillen, John

AU - McNeilly, F.

AU - Duffy, Catherine

AU - McMenamy, Michael

AU - McNair, I.

AU - Hjertner, Bernt

AU - Millar, Andrena

AU - Mckay, K.

AU - Lagan, P.

AU - Adair, Brian

AU - Allan, Gordon

PY - 2011/8/26

Y1 - 2011/8/26

N2 - We report the isolation in cell cultures of two novel bocavirus species in pigs from farms in Northern Ireland with clinical postweaning multisystemic wasting syndrome (PMWS). We have designated the isolates as porcine bocavirus-3 (PBoV3) and porcine bocavirus-4 (PBoV4). To date 5082 and 4125 bps of PBoV3 and PBoV4 have been sequenced, respectively. PBoV3 and PBoV4 show nucleotide homology to other known bocaviruses in swine and other organisms. Open reading frame (ORF) analysis has shown that these viruses have a third small ORF, equivalent to the NP1 ORF that distinguishes the bocaviruses from other parvoviruses.

AB - We report the isolation in cell cultures of two novel bocavirus species in pigs from farms in Northern Ireland with clinical postweaning multisystemic wasting syndrome (PMWS). We have designated the isolates as porcine bocavirus-3 (PBoV3) and porcine bocavirus-4 (PBoV4). To date 5082 and 4125 bps of PBoV3 and PBoV4 have been sequenced, respectively. PBoV3 and PBoV4 show nucleotide homology to other known bocaviruses in swine and other organisms. Open reading frame (ORF) analysis has shown that these viruses have a third small ORF, equivalent to the NP1 ORF that distinguishes the bocaviruses from other parvoviruses.

U2 - 10.1016/j.vetmic.2011.04.013

DO - 10.1016/j.vetmic.2011.04.013

M3 - Article

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JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1-2

ER -

Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland

Abstract

ASJC Scopus subject areas

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