Isolation of antigenic proteins from erythrocytes parasitised with Babesia divergens and a comparison of their immunising potential

S. M. Taylor*, C. T. Elliott, J. Kenny

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Soluble proteins protein in the supernatant prepared from a sonicated lysate of concentrated erythorcytes infected with Babesia divergens were separated by isoelectric focussing into acidic and non acidic fractions. The acidic fraction was further separated by passage through Concanavalin A Sepharose (Con A) into two fractions, the first (Fraction 2) consisting of proteins plus one glycoprotein which did not bind to Con A, the second of glycoproteins (Fraction 1) which eluted after binding to Con A. One group of six cows was treated with three injections of Fraction 2 with incomplete Freunds Adjuvant (IFA); two other groups received the same number of injections of Fraction 1, one with IFA, the other with glucan as adjuvant. These cows plus an untreated control group were infected with 108 erythrocytes infected with a heterologous isolate of B. divergens. Only the group treated with Fraction 2 (Group 1) resisted lethal multiplication of the parasite. There were significant differences in magnitude of parasitaemia, reduction of packed cell volume and antibody titres between Group 1 and the other groups.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalVeterinary Parasitology
Volume21
Issue number2
DOIs
Publication statusPublished - 01 Jan 1986
Externally publishedYes

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Babesia divergens
Babesia
adjuvants
erythrocytes
Erythrocytes
concanavalin A
agarose
glycoproteins
Glycoproteins
injection
cows
Injections
Proteins
Glucans
Parasitemia
proteins
parasitemia
Isoelectric Focusing
glucans
Cell Size

Cite this

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title = "Isolation of antigenic proteins from erythrocytes parasitised with Babesia divergens and a comparison of their immunising potential",
abstract = "Soluble proteins protein in the supernatant prepared from a sonicated lysate of concentrated erythorcytes infected with Babesia divergens were separated by isoelectric focussing into acidic and non acidic fractions. The acidic fraction was further separated by passage through Concanavalin A Sepharose (Con A) into two fractions, the first (Fraction 2) consisting of proteins plus one glycoprotein which did not bind to Con A, the second of glycoproteins (Fraction 1) which eluted after binding to Con A. One group of six cows was treated with three injections of Fraction 2 with incomplete Freunds Adjuvant (IFA); two other groups received the same number of injections of Fraction 1, one with IFA, the other with glucan as adjuvant. These cows plus an untreated control group were infected with 108 erythrocytes infected with a heterologous isolate of B. divergens. Only the group treated with Fraction 2 (Group 1) resisted lethal multiplication of the parasite. There were significant differences in magnitude of parasitaemia, reduction of packed cell volume and antibody titres between Group 1 and the other groups.",
author = "Taylor, {S. M.} and Elliott, {C. T.} and J. Kenny",
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Isolation of antigenic proteins from erythrocytes parasitised with Babesia divergens and a comparison of their immunising potential. / Taylor, S. M.; Elliott, C. T.; Kenny, J.

In: Veterinary Parasitology, Vol. 21, No. 2, 01.01.1986, p. 99-105.

Research output: Contribution to journalArticle

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