Isolation of Atlantic salmon (Salmo salar L.) cytochrome c oxidase subunit II gene (coxII)

G. Hardiman; J. Wolff; J. Peden; F. Gannon

doi:10.1111/j.1439-0426.1994.tb00143.x

Isolation of Atlantic salmon (Salmo salar L.) cytochrome c oxidase subunit II gene (coxII)

G. Hardiman
, J. Wolff
, J. Peden
, F. Gannon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Screening an Atlantic salmon (Salmo salar L.) salmon kidney cDNA library with total cDNA led to the isolation of a clone which hybridised strongly to both salmon kidney and spleen total cDNA probes. Analysis of the sequence identified the cDNA as the gene coding for subunit II of cytochrorne c oxidase (coxII). Northern blot studies revealed a transcript of approximately 700 bp in liver poly A+ mRNA. The DNA sequence of the Atlantic salmon coxII gene employs the mammalian mitochondrial genetic code and is strongly conserved when compared to other species.

Original language	English
Pages (from-to)	64-68
Number of pages	5
Journal	Journal of Applied Ichthyology
Volume	10
Issue number	1
DOIs	https://doi.org/10.1111/j.1439-0426.1994.tb00143.x
Publication status	Published - 01 Jan 1994
Externally published	Yes

ASJC Scopus subject areas

Aquatic Science

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10.1111/j.1439-0426.1994.tb00143.x

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abstract = "Screening an Atlantic salmon (Salmo salar L.) salmon kidney cDNA library with total cDNA led to the isolation of a clone which hybridised strongly to both salmon kidney and spleen total cDNA probes. Analysis of the sequence identified the cDNA as the gene coding for subunit II of cytochrorne c oxidase (coxII). Northern blot studies revealed a transcript of approximately 700 bp in liver poly A+ mRNA. The DNA sequence of the Atlantic salmon coxII gene employs the mammalian mitochondrial genetic code and is strongly conserved when compared to other species.",

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AU - Wolff, J.

AU - Peden, J.

AU - Gannon, F.

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Y1 - 1994/1/1

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AB - Screening an Atlantic salmon (Salmo salar L.) salmon kidney cDNA library with total cDNA led to the isolation of a clone which hybridised strongly to both salmon kidney and spleen total cDNA probes. Analysis of the sequence identified the cDNA as the gene coding for subunit II of cytochrorne c oxidase (coxII). Northern blot studies revealed a transcript of approximately 700 bp in liver poly A+ mRNA. The DNA sequence of the Atlantic salmon coxII gene employs the mammalian mitochondrial genetic code and is strongly conserved when compared to other species.

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ER -

Isolation of Atlantic salmon (Salmo salar L.) cytochrome c oxidase subunit II gene (coxII)

Abstract

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