Isolation of monocytes from human peripheral blood using immuno-affinity expanded-bed adsorption

L.B. Ujam, R.H. Clemmitt, Susan Clarke, R.A. Brooks, N. Rushton, H.A. Chase

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A novel technique for the separation of monocytes from human peripheral blood preparations has been developed. The technique is based on the use of expanded-bed adsorption and a solid perfluorocarbon derivatized with avidin or streptavidin for the indirect positive or negative capture of cells labeled with biotinylated monoclonal antibodies. The perfluorocarbon support was prepared and characterized and the contactor design and operating conditions, that enable cells to be selectively isolated, were investigated. Experiments consisted of applying an immunolabeled pulse of 1 x 10(8) peripheral blood mononuclear cells (PBMCs), isolated by density gradient centrifugation, directly onto a refrigerated expanded bed. The major cell types remaining were T-lymphocytes, B-lymphocytes, and monocytes. Monocytes could be positively adsorbed, following labeling with anti-CD14 mAb, with a clearance of up to 89% and a depletion factor of 7.6. They could also be
Original languageEnglish
Pages (from-to)554-566
Number of pages13
JournalBiotechnology and Bioengineering
Volume83
Issue number5
DOIs
Publication statusPublished - 20 Jun 2003

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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