Isolation of promoter for cytosolic phospholipase A2 (cPLA2)

A Tay, P Maxwell, Z Li, H Goldberg, K Skorecki, Alexander Maxwell

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

Cytosolic phospholipase A2 (cPLA2) releases arachidonic acid from membrane phospholipids and is believed to be the rate-limiting enzyme in the arachidonic acid pathway. We report herein the isolation of a 3 kb fragment of rodent genomic DNA containing part of the first intron, the first exon and 5'-flanking sequence. The start site of transcription was mapped by 5'-rapid amplification of cDNA ends and corroborated by ribonuclease protection assay. The gene has a TATAless promoter with no classical Sp1 binding sites or initiator element. A microsatellite series of CA repeats was noted in the 5'-flanking region of both the rodent and human promoters. Deletion constructs have been analysed for luciferase activity and confirmed promoter activity.
Original languageEnglish
Pages (from-to)345-7
Number of pages3
JournalBiochimica et biophysica acta
Volume1217
Issue number3
Publication statusPublished - 06 Apr 1994

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cytosol
  • DNA
  • Exons
  • Introns
  • Molecular Sequence Data
  • Phospholipases A
  • Phospholipases A2
  • Promoter Regions, Genetic
  • Rats
  • Sequence Alignment

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