Abstract
Cytosolic phospholipase A2 (cPLA2) releases arachidonic acid from membrane phospholipids and is believed to be the rate-limiting enzyme in the arachidonic acid pathway. We report herein the isolation of a 3 kb fragment of rodent genomic DNA containing part of the first intron, the first exon and 5'-flanking sequence. The start site of transcription was mapped by 5'-rapid amplification of cDNA ends and corroborated by ribonuclease protection assay. The gene has a TATAless promoter with no classical Sp1 binding sites or initiator element. A microsatellite series of CA repeats was noted in the 5'-flanking region of both the rodent and human promoters. Deletion constructs have been analysed for luciferase activity and confirmed promoter activity.
Original language | English |
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Pages (from-to) | 345-7 |
Number of pages | 3 |
Journal | Biochimica et biophysica acta |
Volume | 1217 |
Issue number | 3 |
Publication status | Published - 06 Apr 1994 |
Keywords
- Amino Acid Sequence
- Animals
- Base Sequence
- Cytosol
- DNA
- Exons
- Introns
- Molecular Sequence Data
- Phospholipases A
- Phospholipases A2
- Promoter Regions, Genetic
- Rats
- Sequence Alignment