TY - CHAP
T1 - Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica
AU - de la Torre-Escudero, Eduardo
AU - Robinson, Mark W
PY - 2020/5/14
Y1 - 2020/5/14
N2 - Proteins secreted by, or displayed on the surface tegument of, trematodes have key functions in the host-parasite interaction. As such, they are often leading targets for diagnostic tests or vaccine candidates. Here we describe methods for the isolation and analysis of soluble secreted proteins (i.e., the secretome) released during in vitro culture of adult Fasciola hepatica. We also describe two methods for the enrichment of proteins displayed on the outer tegumental surface of F. hepatica. These approaches enable downstream identification of the isolated proteins by mass spectrometry-based proteomics.
AB - Proteins secreted by, or displayed on the surface tegument of, trematodes have key functions in the host-parasite interaction. As such, they are often leading targets for diagnostic tests or vaccine candidates. Here we describe methods for the isolation and analysis of soluble secreted proteins (i.e., the secretome) released during in vitro culture of adult Fasciola hepatica. We also describe two methods for the enrichment of proteins displayed on the outer tegumental surface of F. hepatica. These approaches enable downstream identification of the isolated proteins by mass spectrometry-based proteomics.
U2 - 10.1007/978-1-0716-0475-5_3
DO - 10.1007/978-1-0716-0475-5_3
M3 - Chapter (peer-reviewed)
C2 - 32399919
VL - 2137
T3 - Methods in Molecular Biology
SP - 27
EP - 36
BT - Fasciola hepatica: Methods and Protocols
PB - Springer
ER -
