Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica

Eduardo de la Torre-Escudero, Mark W Robinson

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Proteins secreted by, or displayed on the surface tegument of, trematodes have key functions in the host-parasite interaction. As such, they are often leading targets for diagnostic tests or vaccine candidates. Here we describe methods for the isolation and analysis of soluble secreted proteins (i.e., the secretome) released during in vitro culture of adult Fasciola hepatica. We also describe two methods for the enrichment of proteins displayed on the outer tegumental surface of F. hepatica. These approaches enable downstream identification of the isolated proteins by mass spectrometry-based proteomics.

Original languageEnglish
Title of host publicationFasciola hepatica: Methods and Protocols
PublisherSpringer
Pages27-36
Number of pages10
Volume2137
DOIs
Publication statusEarly online date - 14 May 2020

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745

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