Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica

Eduardo de la Torre-Escudero; Mark W Robinson

doi:10.1007/978-1-0716-0475-5_3

Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica

Eduardo de la Torre-Escudero
, Mark W Robinson

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

2 Citations (Scopus)

Abstract

Proteins secreted by, or displayed on the surface tegument of, trematodes have key functions in the host-parasite interaction. As such, they are often leading targets for diagnostic tests or vaccine candidates. Here we describe methods for the isolation and analysis of soluble secreted proteins (i.e., the secretome) released during in vitro culture of adult Fasciola hepatica. We also describe two methods for the enrichment of proteins displayed on the outer tegumental surface of F. hepatica. These approaches enable downstream identification of the isolated proteins by mass spectrometry-based proteomics.

Original language	English
Title of host publication	Fasciola hepatica: Methods and Protocols
Publisher	Springer
Pages	27-36
Number of pages	10
Volume	2137
DOIs	https://doi.org/10.1007/978-1-0716-0475-5_3
Publication status	Early online date - 14 May 2020

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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abstract = "Proteins secreted by, or displayed on the surface tegument of, trematodes have key functions in the host-parasite interaction. As such, they are often leading targets for diagnostic tests or vaccine candidates. Here we describe methods for the isolation and analysis of soluble secreted proteins (i.e., the secretome) released during in vitro culture of adult Fasciola hepatica. We also describe two methods for the enrichment of proteins displayed on the outer tegumental surface of F. hepatica. These approaches enable downstream identification of the isolated proteins by mass spectrometry-based proteomics.",

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AU - Robinson, Mark W

PY - 2020/5/14

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AB - Proteins secreted by, or displayed on the surface tegument of, trematodes have key functions in the host-parasite interaction. As such, they are often leading targets for diagnostic tests or vaccine candidates. Here we describe methods for the isolation and analysis of soluble secreted proteins (i.e., the secretome) released during in vitro culture of adult Fasciola hepatica. We also describe two methods for the enrichment of proteins displayed on the outer tegumental surface of F. hepatica. These approaches enable downstream identification of the isolated proteins by mass spectrometry-based proteomics.

U2 - 10.1007/978-1-0716-0475-5_3

DO - 10.1007/978-1-0716-0475-5_3

M3 - Chapter (peer-reviewed)

C2 - 32399919

VL - 2137

T3 - Methods in Molecular Biology

SP - 27

EP - 36

BT - Fasciola hepatica: Methods and Protocols

PB - Springer

ER -

Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica

Abstract

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UN SDGs

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