Isotope dilution gas chromatography/mass spectrometry method for the determination of methionine sulfoxide in protein

M A Sochaski, A J Jenkins, T J Lyons, S R Thorpe, J W Baynes

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

We have developed a new technique for quantifying methionine sulfoxide (MetSO) in protein to assess levels of oxidative stress in physiological systems. In this procedure, samples are hydrolyzed with methanesulfonic acid (MSA) in order to avoid the conversion of MetSO to methionine (Met) that occurs during hydrolysis of protein in HCl. The hydrolysate is fractionated on a cation exchange column to remove the nonvolatile MSA from amino acids, and the amino acids are then derivatized as their trimethylsilyl esters for analysis by selected ion monitoring-gas chromatography/mass spectrometry. The limit of detection of the assay is 200 pmol of MetSO per analysis, and the interassay coefficient of variation is 5.8%. Compared to current methods, the SIM-GC/MS assay avoids the potential for conversion of Met to MetSO during sample preparation, requires less sample preparation time, has lower variability, and uses mass spectrometry for sensitive and specific analyte detection.
Original languageEnglish
Pages (from-to)4662-7
Number of pages6
JournalAnalytical Chemistry
Volume73
Issue number19
Publication statusPublished - 01 Oct 2001

Keywords

  • Collagen
  • Crystallins
  • Cyanogen Bromide
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Kinetics
  • Lipoproteins, LDL
  • Lysine
  • Methionine
  • Oxidative Stress
  • Proteins
  • Skin

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