Kassinakinin S: A novel histamine-releasing heptadecapeptide from frog (Kassina senegalensis) skin secretion

Tianbao Chen, Cherith N. Reid, Brian Walker, Mei Zhou, Chris Shaw

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Amphibian defensive skin secretions remain a largely untapped resource for the peptide biochemist with an interest in the identification, structural characterization, and precursor cDNA cloning of novel bioactive peptides. Here we report the isolation, structural characterization, functional profiling, and nucleotide sequence of precursor cDNA of a novel histamine-releasing heptadecapeptide, FIPVTLLALHKIKEKLN-amide, from the defensive skin secretion of the African running frog, Kassina senegalensis. This peptide was found to be a potent histamine secretagogue (EC[5][0]=6 µM; maximal release = 25 µM) in a rat peritoneal mast cell model system and was accordingly named kassinakinin S. The open-reading frame of the cDNA encoding prepro-kassinakinin S was found to consist of 71 amino acid residues containing a single copy of kassinakinin S and its glycyl residue amide donor at the C-terminus. Kassinakinin S can thus be added to the growing list of amphibian skin bioactive peptide prototypes.
Original languageEnglish
Pages (from-to)474-480
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number2
DOIs
Publication statusPublished - 18 Nov 2005

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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