KATNAL1 regulation of sertoli cell microtubule dynamics is essential for spermiogenesis and male fertility

Lee B Smith, Laura Milne, Nancy Nelson, Sharon Eddie, Pamela Brown, Nina Atanassova, Moira K O'Bryan, Liza O'Donnell, Danielle Rhodes, Sara Wells, Diane Napper, Patrick Nolan, Zuzanna Lalanne, Michael Cheeseman, Josephine Peters

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Spermatogenesis is a complex process reliant upon interactions between germ cells (GC) and supporting somatic cells. Testicular Sertoli cells (SC) support GCs during maturation through physical attachment, the provision of nutrients, and protection from immunological attack. This role is facilitated by an active cytoskeleton of parallel microtubule arrays that permit transport of nutrients to GCs, as well as translocation of spermatids through the seminiferous epithelium during maturation. It is well established that chemical perturbation of SC microtubule remodelling leads to premature GC exfoliation demonstrating that microtubule remodelling is an essential component of male fertility, yet the genes responsible for this process remain unknown. Using a random ENU mutagenesis approach, we have identified a novel mouse line displaying male-specific infertility, due to a point mutation in the highly conserved ATPase domain of the novel KATANIN p60-related microtubule severing protein Katanin p60 subunit A-like1 (KATNAL1). We demonstrate that Katnal1 is expressed in testicular Sertoli cells (SC) from 15.5 days post-coitum (dpc) and that, consistent with chemical disruption models, loss of function of KATNAL1 leads to male-specific infertility through disruption of SC microtubule dynamics and premature exfoliation of spermatids from the seminiferous epithelium. The identification of KATNAL1 as an essential regulator of male fertility provides a significant novel entry point into advancing our understanding of how SC microtubule dynamics promotes male fertility. Such information will have resonance both for future treatment of male fertility and the development of non-hormonal male contraceptives.

Original languageEnglish
Pages (from-to)e1002697
JournalPLoS Genetics
Volume8
Issue number5
DOIs
Publication statusPublished - 2012

Keywords

  • Adenosine Triphosphatases
  • Animals
  • Cell Line, Tumor
  • Chromosome Mapping
  • Gene Expression
  • Germ Cells
  • Humans
  • Infertility, Male
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Microtubules
  • Mutagenesis
  • Phenotype
  • Polymorphism, Single Nucleotide
  • Seminiferous Epithelium
  • Sertoli Cells
  • Spermatids
  • Spermatogenesis

Fingerprint Dive into the research topics of 'KATNAL1 regulation of sertoli cell microtubule dynamics is essential for spermiogenesis and male fertility'. Together they form a unique fingerprint.

Cite this