Analysis of binding recognition and conformation of biomolecules is of paramount important in understanding of their vital functions in complex biological systems. By enabling sub-wavelength light localization and strong local field enhancement, plasmonic biosensors have become dominant tools used for such analysis owing to their label-free and real-time attributes1,2. However, the plasmonic biosensors are not well-suited to provide information regarding conformation or chemical fingerprint of biomolecules. Here, we show that plasmonic metamaterials, consisting of periodic arrays of artificial split-ring resonators (SRRs)3, can enable capabilities of both sensing and fingerprinting of biomolecules. We demonstrate that by engineering geometry of individual SRRs, localized surface plasmon resonance (LSPR) frequency of the metamaterials could be tuned to visible-near infrared regimes (Vis-NIR) such that they possess high local field enhancement for surface-enhanced Raman scattering spectroscopy (SERS). This will provide the basis for the development of a dual mode label-free conformational-resolving and quantitative detection platform. We present here the ability of each sensing mode to independently detect binding adsorption and to identify different conformational states of Guanine (G)-rich DNA monolayers in different environment milieu. Also shown is the use of the nanosensor for fingerprinting and detection of Arginine-Glycine-Glycine (RGG) peptide binding to the G-quadruplex aptamer. The dual-mode nanosensor will significantly contribute to unraveling the complexes of the conformational dynamics of biomolecules as well as to improving specificity of biodetection assays that the conventional, population-averaged plasmonic biosensors cannot achieve.
|Publication status||Published - 2013|
|Event||The 5th IEEE International Nanoelectronics Conference 2013 (Oral presentation) - Singapore , Singapore|
Duration: 02 Jan 2013 → 04 Jan 2013
|Conference||The 5th IEEE International Nanoelectronics Conference 2013 (Oral presentation)|
|Period||02/01/2013 → 04/01/2013|
Cao, C., Zhang, J., Dodson, S. L., Wen, X., Dao, N. T., Wong , L. M., Wang , S., Phan, A. T., & Xiong, Q. (2013). Label-free Analysis of Conformational Dynamics and Molecular Interaction of Biomolecules by Vis-NIR Metasensor. The 5th IEEE International Nanoelectronics Conference 2013 (Oral presentation), Singapore , Singapore. http://www.inec2013.org/