Label-Free Detection of Single-Base Mismatches in DNA by Surface-Enhanced Raman Spectroscopy

Evanthia Papadopoulou, Steven E. J. Bell

Research output: Contribution to journalArticlepeer-review

128 Citations (Scopus)
208 Downloads (Pure)

Abstract

Singles only: DNA sequences can be induced to spontaneously adsorb to the surfaces of Ag colloids through their nucleotide side chains (see picture). The SERS spectra of these nonspecifically bound strands are sufficiently reproducible that they can be used to identify single-base mismatches in short (25-mer and 23-mer) strands. Subtracting the spectra of different DNA sequences results in difference spectra that contain features corresponding to the exchanged nucleotides.
Original languageEnglish
Pages (from-to)9058-9061
Number of pages4
JournalAngewandte Chemie International Edition
Volume50
Issue number39
Early online date19 Jul 2011
DOIs
Publication statusPublished - 19 Sep 2011

Keywords

  • DNA
  • nanomaterials
  • nucleotides
  • RNA
  • SERS

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

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