Lack of in vitro and in vivo effects of lipopolysaccharide on porcine circovirus type 2 infection

L.T. Fernandes; E. Mateu; M. Sibila; M. Fort; A. Andaluz; F. McNeilly; Gordon Allan; A. Sanchez; J. Segales; L. Stevenson

doi:10.1089/vim.2007.0065

Lack of in vitro and in vivo effects of lipopolysaccharide on porcine circovirus type 2 infection

L.T. Fernandes, E. Mateu, M. Sibila, M. Fort, A. Andaluz, F. McNeilly, Gordon Allan, A. Sanchez, J. Segales, L. Stevenson

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS). The presence of immunostimulating factors or concurrent infections seems to be crucial for PMWS development. Lipopolysaccharide (LPS) is a potent immunological activator and has recently been suggested to enhance PCV2 replication in vitro. This study was designed to evaluate the effects of different LPS products on PCV2 in vitro replication of pulmonary macrophages (PMs), and on the potential ability to trigger PMWS in cesarean-derived, colostrum-deprived (CDCD) PCV2-inoculated piglets. In vitro studies using two different PCV2 isolates (Stoon-1010 and 1452/3) showed the presence of PCV2 antigen within the cytoplasm to a variable degree; PCV2 Stoon-1010 was barely detectable (

Original language	English
Pages (from-to)	541-552
Number of pages	12
Journal	Viral Immunology
Volume	20
Issue number	4
DOIs	https://doi.org/10.1089/vim.2007.0065
Publication status	Published - 01 Dec 2007

ASJC Scopus subject areas

Immunology
Virology

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10.1089/vim.2007.0065

Cite this

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title = "Lack of in vitro and in vivo effects of lipopolysaccharide on porcine circovirus type 2 infection",

abstract = "Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS). The presence of immunostimulating factors or concurrent infections seems to be crucial for PMWS development. Lipopolysaccharide (LPS) is a potent immunological activator and has recently been suggested to enhance PCV2 replication in vitro. This study was designed to evaluate the effects of different LPS products on PCV2 in vitro replication of pulmonary macrophages (PMs), and on the potential ability to trigger PMWS in cesarean-derived, colostrum-deprived (CDCD) PCV2-inoculated piglets. In vitro studies using two different PCV2 isolates (Stoon-1010 and 1452/3) showed the presence of PCV2 antigen within the cytoplasm to a variable degree; PCV2 Stoon-1010 was barely detectable (",

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T1 - Lack of in vitro and in vivo effects of lipopolysaccharide on porcine circovirus type 2 infection

AU - Fernandes, L.T.

AU - Mateu, E.

AU - Sibila, M.

AU - Fort, M.

AU - Andaluz, A.

AU - McNeilly, F.

AU - Allan, Gordon

AU - Sanchez, A.

AU - Segales, J.

AU - Stevenson, L.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS). The presence of immunostimulating factors or concurrent infections seems to be crucial for PMWS development. Lipopolysaccharide (LPS) is a potent immunological activator and has recently been suggested to enhance PCV2 replication in vitro. This study was designed to evaluate the effects of different LPS products on PCV2 in vitro replication of pulmonary macrophages (PMs), and on the potential ability to trigger PMWS in cesarean-derived, colostrum-deprived (CDCD) PCV2-inoculated piglets. In vitro studies using two different PCV2 isolates (Stoon-1010 and 1452/3) showed the presence of PCV2 antigen within the cytoplasm to a variable degree; PCV2 Stoon-1010 was barely detectable (

AB - Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS). The presence of immunostimulating factors or concurrent infections seems to be crucial for PMWS development. Lipopolysaccharide (LPS) is a potent immunological activator and has recently been suggested to enhance PCV2 replication in vitro. This study was designed to evaluate the effects of different LPS products on PCV2 in vitro replication of pulmonary macrophages (PMs), and on the potential ability to trigger PMWS in cesarean-derived, colostrum-deprived (CDCD) PCV2-inoculated piglets. In vitro studies using two different PCV2 isolates (Stoon-1010 and 1452/3) showed the presence of PCV2 antigen within the cytoplasm to a variable degree; PCV2 Stoon-1010 was barely detectable (

U2 - 10.1089/vim.2007.0065

DO - 10.1089/vim.2007.0065

M3 - Article

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JO - Viral Immunology

JF - Viral Immunology

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ER -

Lack of in vitro and in vivo effects of lipopolysaccharide on porcine circovirus type 2 infection

Abstract

ASJC Scopus subject areas

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