Laser Surface Treatment of Polyamide and NiTi Alloy and the Effects on Mesenchymal Stem Cell Response

David  Waugh; Jonathan  Lawrence; Pratik Shukla; Chi Wai Chan; Issam  Hussain; Hau-Chung Man; Graham Smith

doi:http://dx.doi.org/10.1117/12.2182432

Laser Surface Treatment of Polyamide and NiTi Alloy and the Effects on Mesenchymal Stem Cell Response

David Waugh, Jonathan Lawrence, Pratik Shukla, Chi Wai Chan, Issam Hussain, Hau-Chung Man, Graham Smith

School of Mechanical and Aerospace Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

Original language	English
Title of host publication	Laser Surface Treatment of Polyamide and NiTi Alloy and the Effects on Mesenchymal Stem Cell Response
Publisher	SPIE Proceeding
DOIs	https://doi.org/10.1117/12.2182432
Publication status	Published - Jul 2015
Event	Industrial Laser Applications Symposium : ILAS 2015 - Mike Green; Cath Rose, Kenilworth, United Kingdom Duration: 17 Mar 2015 → 17 Mar 2015 http://ilas2015.co.uk/

Conference

Conference	Industrial Laser Applications Symposium
Abbreviated title	ILAS 2015
Country	United Kingdom
City	Kenilworth
Period	17/03/2015 → 17/03/2015
Internet address	http://ilas2015.co.uk/

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title = "Laser Surface Treatment of Polyamide and NiTi Alloy and the Effects on Mesenchymal Stem Cell Response",

abstract = "Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work. {\textcopyright} (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.",

author = "David Waugh and Jonathan Lawrence and Pratik Shukla and Chan, {Chi Wai} and Issam Hussain and Hau-Chung Man and Graham Smith",

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language = "English",

booktitle = "Laser Surface Treatment of Polyamide and NiTi Alloy and the Effects on Mesenchymal Stem Cell Response",

publisher = "SPIE Proceeding",

note = "Industrial Laser Applications Symposium : ILAS 2015, ILAS 2015 ; Conference date: 17-03-2015 Through 17-03-2015",

url = "http://ilas2015.co.uk/",

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Waugh, D, Lawrence, J, Shukla, P, Chan, CW, Hussain, I, Man, H-C & Smith, G 2015, Laser Surface Treatment of Polyamide and NiTi Alloy and the Effects on Mesenchymal Stem Cell Response. in Laser Surface Treatment of Polyamide and NiTi Alloy and the Effects on Mesenchymal Stem Cell Response. SPIE Proceeding, Industrial Laser Applications Symposium , Kenilworth, United Kingdom, 17/03/2015. https://doi.org/10.1117/12.2182432

Laser Surface Treatment of Polyamide and NiTi Alloy and the Effects on Mesenchymal Stem Cell Response. / Waugh, David ; Lawrence, Jonathan ; Shukla, Pratik; Chan, Chi Wai; Hussain, Issam ; Man, Hau-Chung; Smith, Graham.

Laser Surface Treatment of Polyamide and NiTi Alloy and the Effects on Mesenchymal Stem Cell Response. SPIE Proceeding, 2015.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Waugh, David

AU - Lawrence, Jonathan

AU - Shukla, Pratik

AU - Chan, Chi Wai

AU - Hussain, Issam

AU - Man, Hau-Chung

AU - Smith, Graham

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N2 - Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

AB - Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

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