Lepiniopsis ternatensis sap stimulates fibroblast proliferation and down regulates macrophage TNF-α secretion

Rachael L. Moses; Jordanna Dally; Fionnuala T. Lundy; Moses Langat; Robert Kiapranis; Anthony G. Tsolaki; Ryan Moseley; Thomas A.K. Prescott

doi:10.1016/j.fitote.2020.104478

Lepiniopsis ternatensis sap stimulates fibroblast proliferation and down regulates macrophage TNF-α secretion

Rachael L. Moses, Jordanna Dally, Fionnuala T. Lundy, Moses Langat, Robert Kiapranis, Anthony G. Tsolaki, Ryan Moseley, Thomas A.K. Prescott^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The sap of the tree Lepiniopsis ternatensis is used as a topical treatment for cutaneous leg ulcers in Papua New Guinea. This study, which is the first investigation of this medicinal plant, examines the effect of the sap on wound healing biology using human-derived primary cell lines. NMR spectra from 1D and 2D experiments revealed the sap to contain a single major component, identified as the polyphenol, trifucol. The sap significantly increased the proliferation of dermal fibroblasts at just 1.3 μg/ml, without influencing keratinocytes, suggesting a fibroblast-specific mechanism of stimulation. It also significantly inhibited TNF-α secretion by pro-inflammatory M1 macrophages, but not from neutrophils, at 130 μg/ml. The low toxicity of the sap towards dermal cells along with its fibroblast stimulation activity and downregulation of macrophage TNF-α makes it a potentially attractive agent to promote dermal wound healing in chronic non-healing ulcers.

Original language	English
Article number	104478
Journal	Fitoterapia
Volume	141
Early online date	15 Jan 2020
DOIs	https://doi.org/10.1016/j.fitote.2020.104478
Publication status	Published - Mar 2020

Bibliographical note

Funding Information:
Funding for this work was provided by the Christensen Fund. We are grateful to David Middleton and Marie Briggs for botanical identification and Catherine Fulton for assistance with microbiology. We would also like to acknowledge the help provided by members of the Umbi village in New Britain. This work was funded by a grant from the Christensen Fund for Melanesia 2016-8040.

Funding Information:
Funding for this work was provided by the Christensen Fund . We are grateful to David Middleton and Marie Briggs for botanical identification and Catherine Fulton for assistance with microbiology. We would also like to acknowledge the help provided by members of the Umbi village in New Britain.

Publisher Copyright:
© 2020

Keywords

Fibroblast
Lepiniopsis ternatensis
Papua New Guinea
TNF-α
Trifucol
Wound healing

ASJC Scopus subject areas

Pharmacology
Drug Discovery

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Cite this

@article{5ed1fcce505f408694922487432b62bb,

title = "Lepiniopsis ternatensis sap stimulates fibroblast proliferation and down regulates macrophage TNF-α secretion",

abstract = "The sap of the tree Lepiniopsis ternatensis is used as a topical treatment for cutaneous leg ulcers in Papua New Guinea. This study, which is the first investigation of this medicinal plant, examines the effect of the sap on wound healing biology using human-derived primary cell lines. NMR spectra from 1D and 2D experiments revealed the sap to contain a single major component, identified as the polyphenol, trifucol. The sap significantly increased the proliferation of dermal fibroblasts at just 1.3 μg/ml, without influencing keratinocytes, suggesting a fibroblast-specific mechanism of stimulation. It also significantly inhibited TNF-α secretion by pro-inflammatory M1 macrophages, but not from neutrophils, at 130 μg/ml. The low toxicity of the sap towards dermal cells along with its fibroblast stimulation activity and downregulation of macrophage TNF-α makes it a potentially attractive agent to promote dermal wound healing in chronic non-healing ulcers.",

keywords = "Fibroblast, Lepiniopsis ternatensis, Papua New Guinea, TNF-α, Trifucol, Wound healing",

author = "Moses, {Rachael L.} and Jordanna Dally and Lundy, {Fionnuala T.} and Moses Langat and Robert Kiapranis and Tsolaki, {Anthony G.} and Ryan Moseley and Prescott, {Thomas A.K.}",

note = "Funding Information: Funding for this work was provided by the Christensen Fund. We are grateful to David Middleton and Marie Briggs for botanical identification and Catherine Fulton for assistance with microbiology. We would also like to acknowledge the help provided by members of the Umbi village in New Britain. This work was funded by a grant from the Christensen Fund for Melanesia 2016-8040. Funding Information: Funding for this work was provided by the Christensen Fund . We are grateful to David Middleton and Marie Briggs for botanical identification and Catherine Fulton for assistance with microbiology. We would also like to acknowledge the help provided by members of the Umbi village in New Britain. Publisher Copyright: {\textcopyright} 2020",

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AU - Dally, Jordanna

AU - Lundy, Fionnuala T.

AU - Langat, Moses

AU - Kiapranis, Robert

AU - Tsolaki, Anthony G.

AU - Moseley, Ryan

AU - Prescott, Thomas A.K.

N1 - Funding Information: Funding for this work was provided by the Christensen Fund. We are grateful to David Middleton and Marie Briggs for botanical identification and Catherine Fulton for assistance with microbiology. We would also like to acknowledge the help provided by members of the Umbi village in New Britain. This work was funded by a grant from the Christensen Fund for Melanesia 2016-8040. Funding Information: Funding for this work was provided by the Christensen Fund . We are grateful to David Middleton and Marie Briggs for botanical identification and Catherine Fulton for assistance with microbiology. We would also like to acknowledge the help provided by members of the Umbi village in New Britain. Publisher Copyright: © 2020

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N2 - The sap of the tree Lepiniopsis ternatensis is used as a topical treatment for cutaneous leg ulcers in Papua New Guinea. This study, which is the first investigation of this medicinal plant, examines the effect of the sap on wound healing biology using human-derived primary cell lines. NMR spectra from 1D and 2D experiments revealed the sap to contain a single major component, identified as the polyphenol, trifucol. The sap significantly increased the proliferation of dermal fibroblasts at just 1.3 μg/ml, without influencing keratinocytes, suggesting a fibroblast-specific mechanism of stimulation. It also significantly inhibited TNF-α secretion by pro-inflammatory M1 macrophages, but not from neutrophils, at 130 μg/ml. The low toxicity of the sap towards dermal cells along with its fibroblast stimulation activity and downregulation of macrophage TNF-α makes it a potentially attractive agent to promote dermal wound healing in chronic non-healing ulcers.

AB - The sap of the tree Lepiniopsis ternatensis is used as a topical treatment for cutaneous leg ulcers in Papua New Guinea. This study, which is the first investigation of this medicinal plant, examines the effect of the sap on wound healing biology using human-derived primary cell lines. NMR spectra from 1D and 2D experiments revealed the sap to contain a single major component, identified as the polyphenol, trifucol. The sap significantly increased the proliferation of dermal fibroblasts at just 1.3 μg/ml, without influencing keratinocytes, suggesting a fibroblast-specific mechanism of stimulation. It also significantly inhibited TNF-α secretion by pro-inflammatory M1 macrophages, but not from neutrophils, at 130 μg/ml. The low toxicity of the sap towards dermal cells along with its fibroblast stimulation activity and downregulation of macrophage TNF-α makes it a potentially attractive agent to promote dermal wound healing in chronic non-healing ulcers.

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KW - Papua New Guinea

KW - TNF-α

KW - Trifucol

KW - Wound healing

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DO - 10.1016/j.fitote.2020.104478

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JO - Fitoterapia

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SN - 0367-326X

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Lepiniopsis ternatensis sap stimulates fibroblast proliferation and down regulates macrophage TNF-α secretion

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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