Localised genetic heterogeneity provides a novel mode of evolution in dsDNA phages

Damian J. Magill, Phillip A. Kucher, Victor N. Krylov, Elena A. Pleteneva, John P. Quinn, Leonid A. Kulakov*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)
162 Downloads (Pure)

Abstract

The Red Queen hypothesis posits that antagonistic co-evolution between interacting species results in recurrent natural selection via constant cycles of adaptation and counter-adaptation. Interactions such as these are at their most profound in host-parasite systems, with bacteria and their viruses providing the most intense of battlefields. Studies of bacteriophage evolution thus provide unparalleled insight into the remarkable elasticity of living entities. Here, we report a novel phenomenon underpinning the evolutionary trajectory of a group of dsDNA bacteriophages known as the phiKMVviruses. Employing deep next generation sequencing (NGS) analysis of nucleotide polymorphisms we discovered that this group of viruses generates enhanced intraspecies heterogeneity in their genomes. Our results show the localisation of variants to genes implicated in adsorption processes, as well as variation of the frequency and distribution of SNPs within and between members of the phiKMVviruses. We link error-prone DNA polymerase activity to the generation of variants. Critically, we show trans-activity of this phenomenon (the ability of a phiKMVvirus to dramatically increase genetic variability of a co-infecting phage), highlighting the potential of phages exhibiting such capabilities to influence the evolutionary path of other viruses on a global scale.

Original languageEnglish
Article number13731
JournalScientific Reports
Volume7
Issue number1
Early online date23 Oct 2017
DOIs
Publication statusEarly online date - 23 Oct 2017

ASJC Scopus subject areas

  • General

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