Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina

Nuseibah H. AL Qtaish; Ilia Villate-Beitia; Idoia Gallego; Gema Martínez-Navarrete; Cristina Soto-Sánchez; Myriam Sainz-Ramos; Tania B. Lopez-Mendez; Alejandro J. Paredes; Francisco Javier Chichón; Noelia Zamarreño; Eduardo Fernández; Gustavo Puras; José Luis Pedraz

doi:10.1016/j.ijpharm.2023.122968

Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina

Nuseibah H. AL Qtaish, Ilia Villate-Beitia, Idoia Gallego, Gema Martínez-Navarrete, Cristina Soto-Sánchez, Myriam Sainz-Ramos, Tania B. Lopez-Mendez, Alejandro J. Paredes, Francisco Javier Chichón, Noelia Zamarreño, Eduardo Fernández, Gustavo Puras^*, José Luis Pedraz^*

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

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Abstract

Nanodiamonds were combined with niosome, and resulting formulations were named as nanodiasomes, which were evaluated in terms of physicochemical features, cellular internalization, cell viability and transfection efficiency both in in vitro and in in vivo conditions. Such parameters were analyzed at 4 and 25 ^°C, and at 15 and 30 days after their elaboration. Nanodiasomes showed a particle size of 128 nm that was maintained over time inside the ± 10% of deviation, unless after 30 days of storage at 25 °C. Something similar occurred with the initial zeta potential value, 35.2 mV, being both formulations more stable at 4 °C. The incorporation of nanodiamonds into niosomes resulted in a 4-fold increase of transfection efficiency that was maintained over time at 4 and 25 °C. In vivo studies reported high transgene expression of nanodiasomes after subretinal and intravitreal administration in mice, when injected freshly prepared and after 30 days of storage at 4 °C.

Original language	English
Article number	122968
Journal	International Journal of Pharmaceutics
Volume	639
Early online date	28 Apr 2023
DOIs	https://doi.org/10.1016/j.ijpharm.2023.122968
Publication status	Published - 25 May 2023

Bibliographical note

Funding Information:
This work was supported by the Basque Country Government (Consolidated Groups, IT1448-22 and by CIBER -Consorcio Centro de Investigación Biomédica en Red- CB06/01/1028, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación. Authors wish to thank: ICTS “NANBIOSIS”, specifically the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) for the intellectual and technical assistance. Authors also thank SGIker (UPV/EHU) for technical and human support. Authors acknowledge Rocio Arranz, access to the cryoEM CSIC facility in the context of the CRIOMECORR project (ESFRI-2019-01-CSIC-16). I.V.B. thanks the University of the Basque Country (UPV/EHU) for the granted postdoctoral fellowship (call for the Specialization of Doctor Researcher Personnel of the UPV/EHU, grant reference: ESPDOC19/47). M.S.R. thanks the University of the Basque Country (UPV/EHU) for the granted pre-doctoral fellowship (PIF17/79).

Funding Information:
This work was supported by the Basque Country Government (Consolidated Groups, IT1448-22 and by CIBER -Consorcio Centro de Investigación Biomédica en Red- CB06/01/1028, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación. Authors wish to thank: ICTS “NANBIOSIS”, specifically the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) for the intellectual and technical assistance. Authors also thank SGIker (UPV/EHU) for technical and human support. Authors acknowledge Rocio Arranz, access to the cryoEM CSIC facility in the context of the CRIOMECORR project (ESFRI-2019-01-CSIC-16). I.V.B. thanks the University of the Basque Country (UPV/EHU) for the granted postdoctoral fellowship (call for the Specialization of Doctor Researcher Personnel of the UPV/EHU, grant reference: ESPDOC19/47). M.S.R. thanks the University of the Basque Country (UPV/EHU) for the granted pre-doctoral fellowship (PIF17/79).

Publisher Copyright:
© 2023 The Author(s)

Keywords

Gene delivery
Nanodiamond
Niosome
Non-viral
Retina
Stability

ASJC Scopus subject areas

Pharmaceutical Science

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Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina
Copyright 2023 the authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 10.2 MBLicence: CC BY

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AL Qtaish, N. H., Villate-Beitia, I., Gallego, I., Martínez-Navarrete, G., Soto-Sánchez, C., Sainz-Ramos, M., Lopez-Mendez, T. B., Paredes, A. J., Javier Chichón, F., Zamarreño, N., Fernández, E., Puras, G., & Pedraz, J. L. (2023). Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina. International Journal of Pharmaceutics, 639, Article 122968. https://doi.org/10.1016/j.ijpharm.2023.122968

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title = "Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina",

abstract = "Nanodiamonds were combined with niosome, and resulting formulations were named as nanodiasomes, which were evaluated in terms of physicochemical features, cellular internalization, cell viability and transfection efficiency both in in vitro and in in vivo conditions. Such parameters were analyzed at 4 and 25 °C, and at 15 and 30 days after their elaboration. Nanodiasomes showed a particle size of 128 nm that was maintained over time inside the ± 10% of deviation, unless after 30 days of storage at 25 °C. Something similar occurred with the initial zeta potential value, 35.2 mV, being both formulations more stable at 4 °C. The incorporation of nanodiamonds into niosomes resulted in a 4-fold increase of transfection efficiency that was maintained over time at 4 and 25 °C. In vivo studies reported high transgene expression of nanodiasomes after subretinal and intravitreal administration in mice, when injected freshly prepared and after 30 days of storage at 4 °C.",

keywords = "Gene delivery, Nanodiamond, Niosome, Non-viral, Retina, Stability",

author = "{AL Qtaish}, {Nuseibah H.} and Ilia Villate-Beitia and Idoia Gallego and Gema Mart{\'i}nez-Navarrete and Cristina Soto-S{\'a}nchez and Myriam Sainz-Ramos and Lopez-Mendez, {Tania B.} and Paredes, {Alejandro J.} and {Javier Chich{\'o}n}, Francisco and Noelia Zamarre{\~n}o and Eduardo Fern{\'a}ndez and Gustavo Puras and Pedraz, {Jos{\'e} Luis}",

note = "Funding Information: This work was supported by the Basque Country Government (Consolidated Groups, IT1448-22 and by CIBER -Consorcio Centro de Investigaci{\'o}n Biom{\'e}dica en Red- CB06/01/1028, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovaci{\'o}n. Authors wish to thank: ICTS “NANBIOSIS”, specifically the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) for the intellectual and technical assistance. Authors also thank SGIker (UPV/EHU) for technical and human support. Authors acknowledge Rocio Arranz, access to the cryoEM CSIC facility in the context of the CRIOMECORR project (ESFRI-2019-01-CSIC-16). I.V.B. thanks the University of the Basque Country (UPV/EHU) for the granted postdoctoral fellowship (call for the Specialization of Doctor Researcher Personnel of the UPV/EHU, grant reference: ESPDOC19/47). M.S.R. thanks the University of the Basque Country (UPV/EHU) for the granted pre-doctoral fellowship (PIF17/79). Funding Information: This work was supported by the Basque Country Government (Consolidated Groups, IT1448-22 and by CIBER -Consorcio Centro de Investigaci{\'o}n Biom{\'e}dica en Red- CB06/01/1028, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovaci{\'o}n. Authors wish to thank: ICTS “NANBIOSIS”, specifically the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) for the intellectual and technical assistance. Authors also thank SGIker (UPV/EHU) for technical and human support. Authors acknowledge Rocio Arranz, access to the cryoEM CSIC facility in the context of the CRIOMECORR project (ESFRI-2019-01-CSIC-16). I.V.B. thanks the University of the Basque Country (UPV/EHU) for the granted postdoctoral fellowship (call for the Specialization of Doctor Researcher Personnel of the UPV/EHU, grant reference: ESPDOC19/47). M.S.R. thanks the University of the Basque Country (UPV/EHU) for the granted pre-doctoral fellowship (PIF17/79). Publisher Copyright: {\textcopyright} 2023 The Author(s)",

year = "2023",

month = may,

day = "25",

doi = "10.1016/j.ijpharm.2023.122968",

language = "English",

volume = "639",

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AL Qtaish, NH, Villate-Beitia, I, Gallego, I, Martínez-Navarrete, G, Soto-Sánchez, C, Sainz-Ramos, M, Lopez-Mendez, TB, Paredes, AJ, Javier Chichón, F, Zamarreño, N, Fernández, E, Puras, G & Pedraz, JL 2023, 'Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina', International Journal of Pharmaceutics, vol. 639, 122968. https://doi.org/10.1016/j.ijpharm.2023.122968

TY - JOUR

T1 - Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina

AU - AL Qtaish, Nuseibah H.

AU - Villate-Beitia, Ilia

AU - Gallego, Idoia

AU - Martínez-Navarrete, Gema

AU - Soto-Sánchez, Cristina

AU - Sainz-Ramos, Myriam

AU - Lopez-Mendez, Tania B.

AU - Paredes, Alejandro J.

AU - Javier Chichón, Francisco

AU - Zamarreño, Noelia

AU - Fernández, Eduardo

AU - Puras, Gustavo

AU - Pedraz, José Luis

N1 - Funding Information: This work was supported by the Basque Country Government (Consolidated Groups, IT1448-22 and by CIBER -Consorcio Centro de Investigación Biomédica en Red- CB06/01/1028, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación. Authors wish to thank: ICTS “NANBIOSIS”, specifically the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) for the intellectual and technical assistance. Authors also thank SGIker (UPV/EHU) for technical and human support. Authors acknowledge Rocio Arranz, access to the cryoEM CSIC facility in the context of the CRIOMECORR project (ESFRI-2019-01-CSIC-16). I.V.B. thanks the University of the Basque Country (UPV/EHU) for the granted postdoctoral fellowship (call for the Specialization of Doctor Researcher Personnel of the UPV/EHU, grant reference: ESPDOC19/47). M.S.R. thanks the University of the Basque Country (UPV/EHU) for the granted pre-doctoral fellowship (PIF17/79). Funding Information: This work was supported by the Basque Country Government (Consolidated Groups, IT1448-22 and by CIBER -Consorcio Centro de Investigación Biomédica en Red- CB06/01/1028, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación. Authors wish to thank: ICTS “NANBIOSIS”, specifically the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) for the intellectual and technical assistance. Authors also thank SGIker (UPV/EHU) for technical and human support. Authors acknowledge Rocio Arranz, access to the cryoEM CSIC facility in the context of the CRIOMECORR project (ESFRI-2019-01-CSIC-16). I.V.B. thanks the University of the Basque Country (UPV/EHU) for the granted postdoctoral fellowship (call for the Specialization of Doctor Researcher Personnel of the UPV/EHU, grant reference: ESPDOC19/47). M.S.R. thanks the University of the Basque Country (UPV/EHU) for the granted pre-doctoral fellowship (PIF17/79). Publisher Copyright: © 2023 The Author(s)

PY - 2023/5/25

Y1 - 2023/5/25

N2 - Nanodiamonds were combined with niosome, and resulting formulations were named as nanodiasomes, which were evaluated in terms of physicochemical features, cellular internalization, cell viability and transfection efficiency both in in vitro and in in vivo conditions. Such parameters were analyzed at 4 and 25 °C, and at 15 and 30 days after their elaboration. Nanodiasomes showed a particle size of 128 nm that was maintained over time inside the ± 10% of deviation, unless after 30 days of storage at 25 °C. Something similar occurred with the initial zeta potential value, 35.2 mV, being both formulations more stable at 4 °C. The incorporation of nanodiamonds into niosomes resulted in a 4-fold increase of transfection efficiency that was maintained over time at 4 and 25 °C. In vivo studies reported high transgene expression of nanodiasomes after subretinal and intravitreal administration in mice, when injected freshly prepared and after 30 days of storage at 4 °C.

AB - Nanodiamonds were combined with niosome, and resulting formulations were named as nanodiasomes, which were evaluated in terms of physicochemical features, cellular internalization, cell viability and transfection efficiency both in in vitro and in in vivo conditions. Such parameters were analyzed at 4 and 25 °C, and at 15 and 30 days after their elaboration. Nanodiasomes showed a particle size of 128 nm that was maintained over time inside the ± 10% of deviation, unless after 30 days of storage at 25 °C. Something similar occurred with the initial zeta potential value, 35.2 mV, being both formulations more stable at 4 °C. The incorporation of nanodiamonds into niosomes resulted in a 4-fold increase of transfection efficiency that was maintained over time at 4 and 25 °C. In vivo studies reported high transgene expression of nanodiasomes after subretinal and intravitreal administration in mice, when injected freshly prepared and after 30 days of storage at 4 °C.

KW - Gene delivery

KW - Nanodiamond

KW - Niosome

KW - Non-viral

KW - Retina

KW - Stability

U2 - 10.1016/j.ijpharm.2023.122968

DO - 10.1016/j.ijpharm.2023.122968

M3 - Article

C2 - 37080363

AN - SCOPUS:85153631058

SN - 0378-5173

VL - 639

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

M1 - 122968

ER -

Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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