Long-term genomic instability in human lymphocytes induced by single-particle irradiation

M.A. Kadhim, S.J. Marsden, A.M. Malcolmson, M. Folkard, D.T. Goodhead, Kevin Prise, B.D. Michael

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

Recent evidence suggests that genomic instability, which is an important step in carcinogenesis, may be important in the effectiveness of radiation as a carcinogen, particularly for high-LET radiations. Understanding the biological effects underpinning the risks associated with low doses of densely ionizing radiations is complicated in experimental systems by the Poisson distribution of particles that ran be delivered, In this study, we report an approach to determine the effect of the lowest possible cellular radiation dose of densely ionizing at particles, that of a single particle traversal. Using microbeam technology and an approach for immobilizing human T-lymphocytes, we have measured the effects of single alpha -particle traversals on the surviving progeny of cells. A significant increase in the proportion of aberrant cells is observed 12-13 population doublings after exposure, with a high level of chromatid-type aberrations, indicative of an instability phenotype, These data suggest that instability may be important in situations where even a single particle traverses human cells. (C) 2001 by Radiation Research Society.
Original languageEnglish
Pages (from-to)122-126
Number of pages5
JournalRadiation Research
Volume155
Issue number1
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

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