Loss of eIF4E phosphorylation engenders depression-like behaviors via selective mRNA translation

Inês S. Amorim, Sonal Kedia, Stella Kouloulia, Konstanze Simbriger, Ilse Gantois, Seyed Mehdi Jafarnejad, Yupeng Li, Agniete Kampaite, Tine Pooters, Nicola Romanò, Christos G. Gkogkas

Research output: Contribution to journalArticle

19 Citations (Scopus)
139 Downloads (Pure)

Abstract

The MAPK/ERK (mitogen-activated protein kinases/extracellular signal-regulated kinase) pathway is a cardinal regulator of synaptic plasticity, learning, and memory in the hippocampus. One of major endpoints of this signaling cascade is the 5′ mRNA cap binding protein eIF4E (eukaryotic Initiation Factor 4E), which is phosphorylated on Ser 209 by MNK (MAPK-interacting protein kinases) and controls mRNA translation. The precise role of phospho-eIF4E in the brain is yet to be determined. Herein, we demonstrate that ablation of eIF4E phosphorylation in male mice (4Eki mice) does not impair long-term spatial or contextual fear memory, or the late phase of LTP. Using unbiased translational profiling in mouse brain, we show that phospho-eIF4E differentially regulates the translation of a subset of mRNAs linked to inflammation, the extracellular matrix, pituitary hormones, and the serotonin pathway. Consequently, 4Eki male mice display exaggerated inflammatory responses and reduced levels of serotonin, concomitant with depression and anxiety-like behaviors. Remarkably, eIF4E phosphorylation is required for the chronic antidepressant action of the selective serotonin reuptake inhibitor fluoxetine. Finally, we propose a novel phospho-eIF4E-dependent translational control mechanism in the brain, via the GAIT complex (gamma IFN activated inhibitor of translation). In summary, our work proposes a novel translational control mechanism involved in the regulation of inflammation and depression, which could be exploited to design novel therapeutics.
Original languageEnglish
Pages (from-to)2118-2133
JournalThe Journal of Neuroscience
Volume38
Issue number8
DOIs
Publication statusPublished - 21 Feb 2018
Externally publishedYes

Fingerprint Dive into the research topics of 'Loss of eIF4E phosphorylation engenders depression-like behaviors via selective mRNA translation'. Together they form a unique fingerprint.

  • Cite this

    Amorim, I. S., Kedia, S., Kouloulia, S., Simbriger, K., Gantois, I., Jafarnejad, S. M., Li, Y., Kampaite, A., Pooters, T., Romanò, N., & Gkogkas, C. G. (2018). Loss of eIF4E phosphorylation engenders depression-like behaviors via selective mRNA translation. The Journal of Neuroscience, 38(8), 2118-2133. https://doi.org/10.1523/JNEUROSCI.2673-17.2018