Low frequency of the TEL/AML1 fusion gene in acute lymphoblastic leukaemia in Spain.

R. García-Sanz; I. Alaejos; A. Orfão; A. Rasillo; M.C. Chillón; M.D. Tabernero; M.V. Mateos; R. López-Pérez; D. González; A. Balanzategui; M. González; J.F. San Miguel; A. Bortolucci

Low frequency of the TEL/AML1 fusion gene in acute lymphoblastic leukaemia in Spain.

R. García-Sanz, I. Alaejos, A. Orfão, A. Rasillo, M.C. Chillón, M.D. Tabernero, M.V. Mateos, R. López-Pérez, D. González, A. Balanzategui, M. González, J.F. San Miguel, A. Bortolucci

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

We report on a series of Spanish patients with acute lymphoblastic leukaemia in whom the t(12;21) [TEL/AML1] translocation could not be identified with two sensitive techniques: reverse transcript-polymerase chain reaction (RT-PCR) and fluorescence in-situ hybridization (FISH). 101 cases were analysed: 38 children (29 B-cell precursor; nine T-cell precursor) and 63 adults (48 B-cell precursor; 15 T-cell precursor). Specific RT-PCR to amplify the TEL/AML1 fusion transcript was negative in all 101 cases. Moreover, all 38 paediatric samples were also negative by interphase FISH analysis for the presence of the TEL/AML1 fusion. These results suggest the existence of geographic/race variations in the genotype of acute lymphoblastic leukaemia (ALL).

Original language	English
Pages (from-to)	667-669
Number of pages	3
Journal	British Journal of Haematology
Volume	107
Issue number	3
Publication status	Published - Dec 1999

Keywords

Adolescent
Adult
Child
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 21
Core Binding Factor Alpha 2 Subunit
Female
Gene Frequency
Humans
In Situ Hybridization, Fluorescence
Male
Neoplasm Proteins
Oncogene Proteins, Fusion
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Reverse Transcriptase Polymerase Chain Reaction
Spain
Translocation, Genetic

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http://www.ncbi.nlm.nih.gov/pubmed/10583273

Cite this

García-Sanz, R., Alaejos, I., Orfão, A., Rasillo, A., Chillón, M. C., Tabernero, M. D., Mateos, M. V., López-Pérez, R., González, D., Balanzategui, A., González, M., San Miguel, J. F., & Bortolucci, A. (1999). Low frequency of the TEL/AML1 fusion gene in acute lymphoblastic leukaemia in Spain. British Journal of Haematology, 107(3), 667-669. http://www.ncbi.nlm.nih.gov/pubmed/10583273

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title = "Low frequency of the TEL/AML1 fusion gene in acute lymphoblastic leukaemia in Spain.",

abstract = "We report on a series of Spanish patients with acute lymphoblastic leukaemia in whom the t(12;21) [TEL/AML1] translocation could not be identified with two sensitive techniques: reverse transcript-polymerase chain reaction (RT-PCR) and fluorescence in-situ hybridization (FISH). 101 cases were analysed: 38 children (29 B-cell precursor; nine T-cell precursor) and 63 adults (48 B-cell precursor; 15 T-cell precursor). Specific RT-PCR to amplify the TEL/AML1 fusion transcript was negative in all 101 cases. Moreover, all 38 paediatric samples were also negative by interphase FISH analysis for the presence of the TEL/AML1 fusion. These results suggest the existence of geographic/race variations in the genotype of acute lymphoblastic leukaemia (ALL).",

keywords = "Adolescent, Adult, Child, Chromosomes, Human, Pair 12, Chromosomes, Human, Pair 21, Core Binding Factor Alpha 2 Subunit, Female, Gene Frequency, Humans, In Situ Hybridization, Fluorescence, Male, Neoplasm Proteins, Oncogene Proteins, Fusion, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Reverse Transcriptase Polymerase Chain Reaction, Spain, Translocation, Genetic",

author = "R. Garc{\'i}a-Sanz and I. Alaejos and A. Orf{\~a}o and A. Rasillo and M.C. Chill{\'o}n and M.D. Tabernero and M.V. Mateos and R. L{\'o}pez-P{\'e}rez and D. Gonz{\'a}lez and A. Balanzategui and M. Gonz{\'a}lez and {San Miguel}, J.F. and A. Bortolucci",

year = "1999",

month = dec,

language = "English",

volume = "107",

pages = "667--669",

journal = "British Journal of Haematology",

issn = "0007-1048",

publisher = "WILEY-BLACKWELL PUBLISHING, INC",

number = "3",

}

García-Sanz, R, Alaejos, I, Orfão, A, Rasillo, A, Chillón, MC, Tabernero, MD, Mateos, MV, López-Pérez, R, González, D, Balanzategui, A, González, M, San Miguel, JF & Bortolucci, A 1999, 'Low frequency of the TEL/AML1 fusion gene in acute lymphoblastic leukaemia in Spain.', British Journal of Haematology, vol. 107, no. 3, pp. 667-669. <http://www.ncbi.nlm.nih.gov/pubmed/10583273>

TY - JOUR

T1 - Low frequency of the TEL/AML1 fusion gene in acute lymphoblastic leukaemia in Spain.

AU - García-Sanz, R.

AU - Alaejos, I.

AU - Orfão, A.

AU - Rasillo, A.

AU - Chillón, M.C.

AU - Tabernero, M.D.

AU - Mateos, M.V.

AU - López-Pérez, R.

AU - González, D.

AU - Balanzategui, A.

AU - González, M.

AU - San Miguel, J.F.

AU - Bortolucci, A.

PY - 1999/12

Y1 - 1999/12

N2 - We report on a series of Spanish patients with acute lymphoblastic leukaemia in whom the t(12;21) [TEL/AML1] translocation could not be identified with two sensitive techniques: reverse transcript-polymerase chain reaction (RT-PCR) and fluorescence in-situ hybridization (FISH). 101 cases were analysed: 38 children (29 B-cell precursor; nine T-cell precursor) and 63 adults (48 B-cell precursor; 15 T-cell precursor). Specific RT-PCR to amplify the TEL/AML1 fusion transcript was negative in all 101 cases. Moreover, all 38 paediatric samples were also negative by interphase FISH analysis for the presence of the TEL/AML1 fusion. These results suggest the existence of geographic/race variations in the genotype of acute lymphoblastic leukaemia (ALL).

AB - We report on a series of Spanish patients with acute lymphoblastic leukaemia in whom the t(12;21) [TEL/AML1] translocation could not be identified with two sensitive techniques: reverse transcript-polymerase chain reaction (RT-PCR) and fluorescence in-situ hybridization (FISH). 101 cases were analysed: 38 children (29 B-cell precursor; nine T-cell precursor) and 63 adults (48 B-cell precursor; 15 T-cell precursor). Specific RT-PCR to amplify the TEL/AML1 fusion transcript was negative in all 101 cases. Moreover, all 38 paediatric samples were also negative by interphase FISH analysis for the presence of the TEL/AML1 fusion. These results suggest the existence of geographic/race variations in the genotype of acute lymphoblastic leukaemia (ALL).

KW - Adolescent

KW - Adult

KW - Child

KW - Chromosomes, Human, Pair 12

KW - Chromosomes, Human, Pair 21

KW - Core Binding Factor Alpha 2 Subunit

KW - Female

KW - Gene Frequency

KW - Humans

KW - In Situ Hybridization, Fluorescence

KW - Male

KW - Neoplasm Proteins

KW - Oncogene Proteins, Fusion

KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Spain

KW - Translocation, Genetic

M3 - Article

VL - 107

SP - 667

EP - 669

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 3

ER -

Low frequency of the TEL/AML1 fusion gene in acute lymphoblastic leukaemia in Spain.

Abstract

Keywords

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