Luminescent lanthanide cyclen-based enzymatic assay capable of diagnosing the onset of catheter-associated urinary tract infections both in solution and within polymeric hydrogels

Esther M. Surender, Samuel J. Bradberry, Sandra A. Bright, Colin P. McCoy, D. Clive Williams, Thorfinnur Gunnlaugsson

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)
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Abstract

Herein we present a supramolecular (delayed luminescent) Eu(III)-based pH-responsive probe/sensor with the ability to detect the urease-mediated hydrolysis of urea in aqueous solution. A series of photophysical titrations show this Eu(III) chelate behaves as an ‘on-off’ luminescent switching probe, with its luminescence being quenched upon urea being enzymatically convert- ed into ammonia and carbon dioxide. Calculation of the rate constant (k) and activation energy (Ea) for this hydrolysis reaction are detailed; the results demonstrate a direct observation of enzymatic activity in solution by the sensor. The potential application of this probe in detecting the onset of catheter-associated urinary tract infections (CAUTIs) is also demonstrated by incorporating 1.Eu into water-permeable hydrogels that can be utilized as an alternative coating for catheters.
Original languageEnglish
Pages (from-to)381-388
Number of pages8
JournalJournal of the American Chemical Society
Early online date07 Dec 2016
DOIs
Publication statusPublished - 2017

Keywords

  • catheter
  • pH
  • lanthanide
  • urease
  • luninescence
  • biofilm
  • hydrogel

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