Lux-biosensor assessment of pH effects on microbial sorption and toxicity of chlorophenols

G M Sinclair, G I Paton, A A Meharg, K Killham

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


Lux-marked bacterial biosensors and a commercial toxicity testing bacterial strain (Microtox) were exposed to 2,4-dichlorophenol (DCP) and the light output response measured. Increasing DCP concentrations caused a decrease in light output in all three biosensors with an order of sensitivity (in terms of luminescence decrease over the DCP concentration range) of Pseudomonas fluorescens <Escherichia coli <Microtox. Adsorption of DCP to E. coli was measured using uniformly ring labelled [14C]DCP and found to be very rapid. The effect of pH on toxicity and adsorption was also investigated. Low pH values increased the amount of DCP adsorbed to the cell and increased the toxicity of DCP.
Original languageEnglish
Pages (from-to)273-8
Number of pages6
JournalFEMS Microbiology Letters
Issue number2
Publication statusPublished - 15 May 1999

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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