Mass spectrometric analysis of glucose-modified ribonuclease

W.E. Cotham, D.J.S. Hinton, T.O. Metz, J.W.C. Brock, Suzanne Thorpe, John Baynes, Jennifer Ames

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

RNase A (1 mM) was incubated with glucose (0.4 M) at 37degreesC for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the major sites of N-epsilon-(carboxymethyl)lysine formation.
Original languageEnglish
Pages (from-to)1426-1427
Number of pages2
JournalBiochemical society transactions
Volume31
Issue number6
Publication statusPublished - Dec 2003

ASJC Scopus subject areas

  • Biochemistry

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