Mass spectrometric analysis of glucose-modified ribonuclease

W.E. Cotham; D.J.S. Hinton; T.O. Metz; J.W.C. Brock; Suzanne Thorpe; John Baynes; Jennifer Ames

Mass spectrometric analysis of glucose-modified ribonuclease

W.E. Cotham, D.J.S. Hinton, T.O. Metz, J.W.C. Brock, Suzanne Thorpe, John Baynes, Jennifer Ames

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

RNase A (1 mM) was incubated with glucose (0.4 M) at 37degreesC for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the major sites of N-epsilon-(carboxymethyl)lysine formation.

Original language	English
Pages (from-to)	1426-1427
Number of pages	2
Journal	Biochemical Society Transactions
Volume	31
Issue number	6
Publication status	Published - Dec 2003

ASJC Scopus subject areas

Biochemistry

Cite this

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title = "Mass spectrometric analysis of glucose-modified ribonuclease",

abstract = "RNase A (1 mM) was incubated with glucose (0.4 M) at 37degreesC for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the major sites of N-epsilon-(carboxymethyl)lysine formation.",

author = "W.E. Cotham and D.J.S. Hinton and T.O. Metz and J.W.C. Brock and Suzanne Thorpe and John Baynes and Jennifer Ames",

year = "2003",

month = dec,

language = "English",

volume = "31",

pages = "1426--1427",

journal = "Biochemical Society Transactions",

issn = "0300-5127",

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TY - JOUR

T1 - Mass spectrometric analysis of glucose-modified ribonuclease

AU - Cotham, W.E.

AU - Hinton, D.J.S.

AU - Metz, T.O.

AU - Brock, J.W.C.

AU - Thorpe, Suzanne

AU - Baynes, John

AU - Ames, Jennifer

PY - 2003/12

Y1 - 2003/12

N2 - RNase A (1 mM) was incubated with glucose (0.4 M) at 37degreesC for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the major sites of N-epsilon-(carboxymethyl)lysine formation.

AB - RNase A (1 mM) was incubated with glucose (0.4 M) at 37degreesC for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the major sites of N-epsilon-(carboxymethyl)lysine formation.

UR - http://www.scopus.com/inward/record.url?scp=0346158345&partnerID=8YFLogxK

M3 - Article

SN - 0300-5127

VL - 31

SP - 1426

EP - 1427

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - 6

ER -

Mass spectrometric analysis of glucose-modified ribonuclease

Abstract

ASJC Scopus subject areas

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