Mass spectrometric analysis of muscle samples to detect potential antibiotic growth promoter misuse in broiler chickens

R. Gibson; K. M. Cooper; D. G. Kennedy; C. T. Elliott

doi:10.1080/19440049.2012.698398

Mass spectrometric analysis of muscle samples to detect potential antibiotic growth promoter misuse in broiler chickens

R. Gibson, K. M. Cooper, D. G. Kennedy, C. T. Elliott

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Mass spectrometric methods were developed and validated for the analysis in chicken muscle of a range of antibiotic growth promoters: spiramycin, tylosin, virginiamycin and bacitracin, and separately for two marker metabolites of carbadox (quinoxaline-2-carboxylic acid and 1,4-bisdesoxycarbadox), and a marker metabolite of olaquindox (3-methyl-quinoxaline-2-carboxylic acid). The use of these compounds as antibiotic growth promoters has been banned by the European Commission. This study aimed to develop methods to detect their residues in muscle samples as a means of checking for the use of these drugs during the rearing of broiler chickens. When fed growth-promoting doses for 6 days, spiramycin (31.4 mu g kg(-1)), tylosin (1.0 mu g kg(-1)), QCA (6.5 mu g kg(-1)), DCBX (71.2 mu g kg(-1)) and MQCA (0.2 mu g kg(-1)) could be detected in the muscle 0 days after the withdrawal of fortified feed. Only spiramycin could consistently be detected beyond a withdrawal period of 1 day. All analytes showed stability commercial cooking process, therefore raw or cooked muscle could be used for monitoring purposes.

Original language	English
Article number	698398
Pages (from-to)	1413-1424
Number of pages	12
Journal	FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
Volume	29
Issue number	9
DOIs	https://doi.org/10.1080/19440049.2012.698398
Publication status	Published - Jun 2012

ASJC Scopus subject areas

Food Science
Health, Toxicology and Mutagenesis
Public Health, Environmental and Occupational Health
Toxicology
Chemistry(all)

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1080/19440049.2012.698398

Cite this

@article{28abd3f0ef4e46988e96f8c048b90bae,

title = "Mass spectrometric analysis of muscle samples to detect potential antibiotic growth promoter misuse in broiler chickens",

abstract = "Mass spectrometric methods were developed and validated for the analysis in chicken muscle of a range of antibiotic growth promoters: spiramycin, tylosin, virginiamycin and bacitracin, and separately for two marker metabolites of carbadox (quinoxaline-2-carboxylic acid and 1,4-bisdesoxycarbadox), and a marker metabolite of olaquindox (3-methyl-quinoxaline-2-carboxylic acid). The use of these compounds as antibiotic growth promoters has been banned by the European Commission. This study aimed to develop methods to detect their residues in muscle samples as a means of checking for the use of these drugs during the rearing of broiler chickens. When fed growth-promoting doses for 6 days, spiramycin (31.4 mu g kg(-1)), tylosin (1.0 mu g kg(-1)), QCA (6.5 mu g kg(-1)), DCBX (71.2 mu g kg(-1)) and MQCA (0.2 mu g kg(-1)) could be detected in the muscle 0 days after the withdrawal of fortified feed. Only spiramycin could consistently be detected beyond a withdrawal period of 1 day. All analytes showed stability commercial cooking process, therefore raw or cooked muscle could be used for monitoring purposes.",

author = "R. Gibson and Cooper, {K. M.} and Kennedy, {D. G.} and Elliott, {C. T.}",

year = "2012",

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}

Mass spectrometric analysis of muscle samples to detect potential antibiotic growth promoter misuse in broiler chickens. / Gibson, R.; Cooper, K. M.; Kennedy, D. G.; Elliott, C. T.

In: FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, Vol. 29, No. 9, 698398, 06.2012, p. 1413-1424.

Research output: Contribution to journal › Article › peer-review

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