Mass spectrometry-driven discovery of neuropeptides mediating nictation behavior of nematodes

Bram Cockx, Sven Van Bael, Rose Boelen, Elke Vandewyer, Heeseung Yang, Tuan Anh Le, Johnathan J Dalzell, Isabel Beets, Christina Ludwig, Junho Lee, Liesbet Temmerman

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
36 Downloads (Pure)


Neuropeptides regulate animal physiology and behavior, making them widely studied targets of functional genetics research. While the field often relies on differential -omics approaches to build hypotheses, no such method exists for neuropeptidomics. It would nonetheless be valuable for studying behaviors suspected to be regulated by neuropeptides, especially when little information is otherwise available. This includes nictation, a phoretic strategy of Caenorhabditis elegans dauers that parallels host-finding strategies of infective juveniles of many pathogenic nematodes. We here developed a targeted peptidomics method for the model organism C. elegans and show that 161 quantified neuropeptides are more abundant in its dauer stage compared to L3 juveniles. Many of these have orthologs in the commercially relevant pathogenic nematode Steinernema carpocapsae, in whose infective juveniles we identified 126 neuropeptides in total. Through further behavioral genetics experiments, we identify flp-7 and flp-11 as novel regulators of nictation. Our work advances knowledge on the genetics of nictation behavior and adds comparative neuropeptidomics as a tool to functional genetics workflows.
Original languageEnglish
Article number100479
JournalMolecular and Cellular Proteomics
Issue number2
Early online date24 Jan 2023
Publication statusPublished - Feb 2023


Dive into the research topics of 'Mass spectrometry-driven discovery of neuropeptides mediating nictation behavior of nematodes'. Together they form a unique fingerprint.

Cite this