Measuring Chromatin Accessibility: ATAC-Seq

Sanjeeb Kumar Sahu, Amitava Basu, Vijay K Tiwari

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Assay for Transposase-Accessible Chromatin using sequencing (ATAC-Seq) is a method to investigate the accessibility of chromatin in a genome-wide fashion. In this chapter, we provide a brief history of the chromatin accessibility field followed by a detailed protocol to perform ATAC-Seq assay. [Abstract copyright: © 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.]
Original languageEnglish
Pages (from-to)105-121
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 12 Aug 2021


  • Transposase
  • Gene regulation
  • ATAC-seq
  • Chromatin accessibility
  • Nucleosome


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