Measuring Chromatin Accessibility: ATAC-Seq

Sanjeeb Kumar Sahu; Amitava Basu; Vijay K Tiwari

doi:10.1007/978-1-0716-1597-3_6

Measuring Chromatin Accessibility: ATAC-Seq

Sanjeeb Kumar Sahu, Amitava Basu, Vijay K Tiwari

Wellcome Wolfson Institute for Experimental Medicine

Research output: Contribution to journal › Article › peer-review

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Abstract

Assay for Transposase-Accessible Chromatin using sequencing (ATAC-Seq) is a method to investigate the accessibility of chromatin in a genome-wide fashion. In this chapter, we provide a brief history of the chromatin accessibility field followed by a detailed protocol to perform ATAC-Seq assay. [Abstract copyright: © 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.]

Original language	English
Pages (from-to)	105-121
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2351
DOIs	https://doi.org/10.1007/978-1-0716-1597-3_6
Publication status	Published - 12 Aug 2021

Keywords

Transposase
Gene regulation
ATAC-seq
Chromatin accessibility
Nucleosome

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abstract = "Assay for Transposase-Accessible Chromatin using sequencing (ATAC-Seq) is a method to investigate the accessibility of chromatin in a genome-wide fashion. In this chapter, we provide a brief history of the chromatin accessibility field followed by a detailed protocol to perform ATAC-Seq assay. [Abstract copyright: {\textcopyright} 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.]",

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author = "Sahu, {Sanjeeb Kumar} and Amitava Basu and Tiwari, {Vijay K}",

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AB - Assay for Transposase-Accessible Chromatin using sequencing (ATAC-Seq) is a method to investigate the accessibility of chromatin in a genome-wide fashion. In this chapter, we provide a brief history of the chromatin accessibility field followed by a detailed protocol to perform ATAC-Seq assay. [Abstract copyright: © 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.]

KW - Transposase

KW - Gene regulation

KW - ATAC-seq

KW - Chromatin accessibility

KW - Nucleosome

U2 - 10.1007/978-1-0716-1597-3_6

DO - 10.1007/978-1-0716-1597-3_6

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JO - Methods in molecular biology (Clifton, N.J.)

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Measuring Chromatin Accessibility: ATAC-Seq

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Keywords

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