Mechanistic studies on human N-acetylgalactosamine kinase

A. Agnew, David Timson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

N-Acetylgalactosamine kinase (GALK2) is a small molecule kinase from the GHMP family which phosphorylates N-acetylgalactosamine at the expense of ATP. Recombinant GALK2 expressed in, and purified from, Escherichia coli was shown to be active with the following kinetic parameters: Michaelis constant for ATP, 14 +/- 3 mu M; Michaelis constant for N-acetylgalactosamine, 40 +/- 14 mu M; and turnover number, 1.0 +/- 0.1 s
Original languageEnglish
Pages (from-to)370-376
Number of pages7
JournalJournal of Enzyme Inhibition and Medicinal Chemistry
Volume25
Issue number3
DOIs
Publication statusPublished - 2010

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology

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