Abstract
N-Acetylgalactosamine kinase (GALK2) is a small molecule kinase from the GHMP family which phosphorylates N-acetylgalactosamine at the expense of ATP. Recombinant GALK2 expressed in, and purified from, Escherichia coli was shown to be active with the following kinetic parameters: Michaelis constant for ATP, 14 +/- 3 mu M; Michaelis constant for N-acetylgalactosamine, 40 +/- 14 mu M; and turnover number, 1.0 +/- 0.1 s
Original language | English |
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Pages (from-to) | 370-376 |
Number of pages | 7 |
Journal | Journal of Enzyme Inhibition and Medicinal Chemistry |
Volume | 25 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2010 |
ASJC Scopus subject areas
- Drug Discovery
- Pharmacology